Ji Min-Kyung, Lee Min-Joo, Park Sang-Won, Lee Kwangmin, Yun Kwi-Dug, Kim Hyun-Seung, Oh Gye-Jeong, Kim Ji-Hyun, Lim Hyun-Pil
J Nanosci Nanotechnol. 2016 Feb;16(2):1656-9. doi: 10.1166/jnn.2016.11924.
The aim of this study was to evaluate the response of Streptococcus mutans (S. mutans) via crystal violet staining assay on titanium surface modified by physical vapor deposition/plasma assisted chemical vapor deposition process. Specimens were divided into the following three groups: polished titanium (control group), titanium modified by DC magnetron sputtering (group TiN-Ti), and titanium modified by plasma nitriding (group N-Ti). Surface characteristics of specimens were observed by using nanosurface 3D optical profiler and field emission scanning electron microscope. Group TiN-Ti showed TiN layer of 1.2 microm in thickness. Group N-Ti was identified as plasma nitriding with X-ray photoelectron spectroscopy. Roughness average (Ra) of all specimens had values < or = 0.2 microm (the threshold Ra), which had no effect on bacterial adhesion. No significant difference of S. mutans adhesion was found between the surfaces of control, TiN-Ti, and N-Ti (P > 0.05). Within the process condition of this study, modified titanium surfaces by DC magnetron sputtering and plasma nitriding did not influence the adhesion of S. mutans.
本研究的目的是通过结晶紫染色试验评估变形链球菌（S. mutans）对经物理气相沉积/等离子体辅助化学气相沉积工艺改性的钛表面的反应。将标本分为以下三组：抛光钛（对照组）、经直流磁控溅射改性的钛（TiN-Ti组）和经等离子体氮化改性的钛（N-Ti组）。使用纳米表面3D光学轮廓仪和场发射扫描电子显微镜观察标本的表面特征。TiN-Ti组显示TiN层厚度为1.2微米。用X射线光电子能谱鉴定N-Ti组为等离子体氮化。所有标本的平均粗糙度（Ra）值≤0.2微米（阈值Ra），这对细菌粘附没有影响。对照组、TiN-Ti组和N-Ti组表面之间变形链球菌的粘附没有显著差异（P>0.05）。在本研究的工艺条件下，经直流磁控溅射和等离子体氮化改性的钛表面不影响变形链球菌的粘附。
