人膀胱癌中LncRNA MIR31HG表达降低。
Decreased expression of LncRNA MIR31HG in human bladder cancer.
作者信息
He Anbang, Chen Zhicong, Mei Hongbing, Liu Yuchen
机构信息
Shenzhen Second People's Hospital, Clinical Medicine College of Anhui Medical University, Shenzhen, Guangdong, China.
Key Laboratory of Medical Reprogramming Technology, Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University Shenzhen, Guangdong, China.
出版信息
Cancer Biomark. 2016 Jul 8;17(2):231-6. doi: 10.3233/CBM-160635.
OBJECTIVE
In this study, we examined the relationships between the expression level of long non-coding RNA MIR31HG in bladder cancer and the clinical characteristics.
METHODS
A total of 55 tissue samples from patients with bladder cancer were collected, and the lncRNA MIR31HG levels in cancer, paired non-cancer tissues and BC cell lines were detected by real-time quantitative RT-PCR (qRT-PCR). The relationships between MIR31HG level and the clinical characteristics were evaluated.
RESULTS
MIR31HG expression was remarkably decreased in bladder cancer tissues compared with adjacent noncancerous tissues (P < 0.05). MIR31HG expression was also significantly down-regulated in four bladder cancer cell lines (P < 0.001). Clinicopathologic analysis revealed that MIR31HG expression was negatively associated with TNM stage (P = 0.010), but not with other clinicopathological characteristics.
CONCLUSIONS
These findings revealed that MIR31HG may function as a cancer-suppressor gene to participate in the bladder cancer carcinogenesis and development.
目的
在本研究中，我们检测了长链非编码RNA MIR31HG在膀胱癌中的表达水平及其与临床特征的关系。
方法
收集了55例膀胱癌患者的组织样本，采用实时定量逆转录聚合酶链反应（qRT-PCR）检测癌组织、配对的癌旁组织及膀胱癌细胞系中lncRNA MIR31HG的水平，并评估MIR31HG水平与临床特征之间的关系。
结果
与癌旁组织相比，膀胱癌组织中MIR31HG的表达显著降低（P < 0.05）。在4种膀胱癌细胞系中，MIR31HG的表达也明显下调（P < 0.001）。临床病理分析显示，MIR31HG的表达与TNM分期呈负相关（P = 0.010），但与其他临床病理特征无关。
结论
这些结果表明，MIR31HG可能作为一种抑癌基因参与膀胱癌的发生和发展。
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