Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University, Shanghai, China; Department of Gastrointestinal Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Department of Colorectal Surgery, Changhai Hospital, Second Military Medical University, Shanghai, China.
Biomed Pharmacother. 2016 Oct;83:496-501. doi: 10.1016/j.biopha.2016.06.058. Epub 2016 Jul 18.
Colorectal cancer (CRC) is the most common type of gastrointestinal cancer. However, up to date, the specific mechanism for CRC proliferation remains unclear. Transcriptional enhancer activator domain 1 (TEAD1) is a transcription factor belongs to the TEAD family, which plays an important role in cancers progression. Here, we investigated the role of TEAD1 in CRC, and found that overexpression of TEAD1 was proved to increase colorectal cancer cells proliferation, whereas, knockdown of TEAD1 reduces the growth of cancer cells by BrdU assay, cell cycle analysis and MTT assay. Furthermore, we performed luciferase assay and chromatin immunoprecipitation assay to investigate the underlying mechanism of TEAD1 in CRC system, and observed that TEAD1 could enhance the expression levels of SP1, by directly binding to its promoter. In summary, we provided evidence for a novel mechanism regulating growth and proliferation in colorectal cancer.
结直肠癌（CRC）是最常见的胃肠道癌症类型。然而，迄今为止，CRC 增殖的确切机制仍不清楚。转录增强激活结构域 1（TEAD1）是转录因子 TEAD 家族的成员，在癌症进展中发挥着重要作用。在这里，我们研究了 TEAD1 在 CRC 中的作用，发现 TEAD1 的过表达被证明可以增加结直肠癌细胞的增殖，而通过 BrdU 测定、细胞周期分析和 MTT 测定，敲低 TEAD1 则减少了癌细胞的生长。此外，我们进行了荧光素酶测定和染色质免疫沉淀测定，以研究 TEAD1 在 CRC 系统中的潜在机制，并观察到 TEAD1 可以通过直接结合其启动子来增强 SP1 的表达水平。总之，我们为调节结直肠癌细胞生长和增殖的新机制提供了证据。
