Kim Dong-Uk, Lee Hyosun, Kim Hyun, Kim Song-Gun, Ka Jong-Ok
Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, 151-742, Republic of Korea.
Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea.
Antonie Van Leeuwenhoek. 2016 Oct;109(10):1397-402. doi: 10.1007/s10482-016-0738-x. Epub 2016 Jul 19.
A Gram-stain negative, white-pigmented bacterial strain, designated D78(T), was isolated from soil obtained from Dokdo in Korea. The strain was observed to be aerobic, asporogenous and rod-shaped. The strain was observed to grow at 15-40 °C (optimum 37 °C), at pH 6.0-8.0 (optimum 7.0-7.5) and in the presence of 0-1 % (w/v) NaCl. Phylogenetically, the strain was found to be closely related to members of the genus Dongia and showed 16S rRNA gene sequence similarity with Dongia mobilis LM22(T) and Dongia rigui 04SU4-P(T) of 94.6 and 94.4 %, respectively. The major fatty acids were identified as C16:0, C19:0 cyclo ω8c and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). Q-10 was identified as the only ubiquinone. The polar lipids profile was found to contain phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid and three unidentified polar lipids. The DNA G+C content of the strain was found to be 54.7 mol%. Based on the phenotypic, genotypic and chemotaxonomic data, strain D78(T) represents a novel species in the genus Dongia, for which the name Dongia soli sp. nov. (=KACC 13941(T) =LMG 29193(T)) is proposed.
从韩国独岛采集的土壤中分离出一株革兰氏染色阴性、产白色色素的细菌菌株，命名为D78(T)。该菌株为需氧菌，不产芽孢，呈杆状。该菌株在15-40°C（最适温度37°C）、pH 6.0-8.0（最适pH 7.0-7.5）以及0-1%（w/v）NaCl存在的条件下生长。系统发育分析表明，该菌株与东氏菌属成员密切相关，其16S rRNA基因序列与运动东氏菌LM22(T)和李氏东氏菌04SU4-P(T)的相似性分别为94.6%和94.4%。主要脂肪酸鉴定为C16:0、C19:0环ω8c以及总和特征8（C18:1 ω7c和/或C18:1 ω6c）。鉴定出Q-10为唯一的泛醌。发现极性脂质谱包含磷脂酰乙醇胺磷脂酰乙醇胺、羟基磷脂酰乙醇胺、磷脂酰甘油、一种未鉴定的氨基脂质和三种未鉴定的极性脂质。该菌株的DNA G+C含量为54.7 mol%。基于表型、基因型和化学分类学数据，菌株D78(T)代表东氏菌属中的一个新物种，为此提出新名称独岛东氏菌（Dongia soli sp. nov.）（=KACC 13941(T)=LMG 29193(T)）。
