Leibniz-Institut DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
Present address: Darwin Bioprospecting Excellence, S.L., Paterna, Valencia, Spain.
Int J Syst Evol Microbiol. 2020 Mar;70(3):1850-1860. doi: 10.1099/ijsem.0.003983.
Two strains of the family were isolated from the rhizosphere of the medicinal plant . Cells of both strains were Gram-stain-negative, motile by means of a single polar flagellum, non-spore-forming, non-capsulated, short rods that divided by binary fission. Colonies were small and white. Strains R5913 and R5959 were oxidase-positive, mesophilic, neutrophilic and grew optimally without NaCl. Both grew under aerobic and microaerophilic conditions and on a limited range of substrates with best results on yeast extract. Major fatty acids were C cyclo 8 and C; in addition, Cω7 was also found as a predominant fatty acid in strain R5913. The major respiratory quinone was ubiquinone 10 (Q-10). The DNA G+C contents of strains R5913 and R5959 were 66.0 and 67.4 mol%, respectively. 16S rRNA gene sequence comparison revealed that the closest relatives (<92 % similarity) of the strains are MCCC 1A02656, CGMCC 1.7660, D78 and 04SU4-P. The two novel strains shared 98.6 % sequence similarity and represent different species on the basis of low average nucleotide identity of their genomes (83.8 %). Based on the combined phenotypic, genomic and phylogenetic investigations, the two strains represent two novel species of a new genus in the family , for which the name gen. nov. is proposed, comprising the type species sp. nov. (type strain R5913=DSM 109816=CECT 9472) and sp. nov. (type strain R5959=DSM 109817=CECT 9620).
从药用植物根际中分离到两株菌，属于 科。两菌株的细胞革兰氏阴性，通过单一极鞭毛运动，不形成孢子，不形成荚膜，短杆状，通过二分分裂分裂。菌落小而白色。菌株 R5913 和 R5959 氧化酶阳性，中温，嗜中性，在没有 NaCl 的情况下最佳生长。两者在需氧和微需氧条件下以及在有限的底物范围内生长，在酵母提取物上生长最好。主要脂肪酸为 C cyclo 8 和 C ；此外，Cω7 也被发现是菌株 R5913 的主要脂肪酸。主要呼吸醌为泛醌 10（Q-10）。菌株 R5913 和 R5959 的 DNA G+C 含量分别为 66.0 和 67.4 mol%。16S rRNA 基因序列比较表明，两株菌的最接近亲缘菌（<92%相似性）为 MCCC 1A02656、CGMCC 1.7660、D78 和 04SU4-P。这两个新菌株的序列相似度为 98.6%，基于其基因组的低平均核苷酸同一性（83.8%），代表不同的物种。基于综合表型、基因组和系统发育研究，这两株菌代表了 科一个新属的两个新种，提议建立一个新属，其模式种为 sp. nov.（模式菌株 R5913=DSM 109816=CECT 9472）和 sp. nov.（模式菌株 R5959=DSM 109817=CECT 9620）。
