Wang Yanan, Zhang Sufang, Pötter Markus, Sun Wenyi, Li Li, Yang Xiaobing, Jiao Xiang, Zhao Zongbao K
Division of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Appl Biochem Biotechnol. 2016 Dec;180(8):1497-1507. doi: 10.1007/s12010-016-2182-9. Epub 2016 Jul 19.
Under nutrient-limited conditions, the red yeast Rhodosporidium toruloides can accumulate neutral lipids, of which the compositional fatty acids are mainly saturated and mono-unsaturated ones with 16 or 18 carbon atoms. To improve the linoleic acid content in the lipids, we enabled galactose-inducible expression of the gene encoding Δ12-fatty acid desaturase (FADS) from Mortierella alpina or Fusarium verticillioides by integration of the corresponding expression cassettes into the genome of R. toruloides haploid and diploid strains. The relative linoleic acid content increased up to fivefold and the final linoleic acid titer reached 1.3 g/L under flask culture conditions. Our results suggested that R. toruloides may be further explored as cell factory for production of high-valued lipids and other fatty acid derivatives as bio-based chemicals and fuels.
在营养受限条件下，红酵母红冬孢酵母（Rhodosporidium toruloides）能够积累中性脂质，其组成脂肪酸主要是含有16或18个碳原子的饱和脂肪酸和单不饱和脂肪酸。为了提高脂质中亚油酸的含量，我们通过将相应的表达盒整合到红冬孢酵母单倍体和二倍体菌株的基因组中，实现了来自高山被孢霉（Mortierella alpina）或轮状镰刀菌（Fusarium verticillioides）的编码Δ12-脂肪酸去饱和酶（FADS）基因的半乳糖诱导表达。在摇瓶培养条件下，亚油酸相对含量增加了五倍，最终亚油酸滴度达到1.3 g/L。我们的结果表明，红冬孢酵母有望进一步开发成为生产高价值脂质及其他脂肪酸衍生物（作为生物基化学品和燃料）的细胞工厂。
