Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China; Biomedicine Key Laboratory of Shaanxi Province, College of Life Science, Northwest University, Xi'an 710069, China.
Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China; Biomedicine Key Laboratory of Shaanxi Province, College of Life Science, Northwest University, Xi'an 710069, China.
J Ethnopharmacol. 2016 Dec 4;193:12-20. doi: 10.1016/j.jep.2016.07.047. Epub 2016 Jul 17.
Menispermum dauricum DC., commonly known as "Bei Dou Gen" (BDG) in China, has been used extensively in folk medicine to treat inflammatory diseases, especially intestinal inflammations such as enteritis and dysentery, and in pharyngitis, tonsillitis, rheumatism and bronchitis. Although previous studies showed that BDG has anti-inflammatory activities, its effects on ulcerative colitis (UC) have not yet been explored.
To investigate the intestinal anti-inflammatory effect of the rhizome extracts of Menispermum dauricum DC. on UC model induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) in mice.
UC in mice was induced by colonic administration with TNBS. BDG (100, 200 and 400mg/kg/day) and sulfasalazine (500mg/kg/day) were administered orally for 7 consecutive days. The inflammatory degree was assessed by gross appearance, macroscopic and histological analysis, and accumulation of myeloperoxidase (MPO) activity. Pro-inflammatory mediators, including tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6, were determined by enzyme-linked immunoassay. The expression of cyclooxygenase (COX)-2 was assessed by immunohistochemical analysis.
Treatment with different doses of BDG significantly ameliorated macroscopic damage and histological changes, reduced the accumulation of MPO activity, depressed serum and colonic tissue levels of TNF-α, IL-1β and IL-6 in a dose-dependent manner. In addition, administration of BDG effectively reduced COX-2 overexpression in colon.
We demonstrated for the first time that BDG possessed marked intestinal anti-inflammatory effect in TNBS induced colitis in mice, which might be related to the reduction of up-regulated productions and expressions of pro-inflammatory mediators, suggesting that it may have beneficial use for the treatment of inflammatory bowel disease.
防己科蝙蝠葛属植物，在中国俗称“北豆根”（BDG），被广泛应用于民间医学来治疗炎症性疾病，特别是肠炎和痢疾等肠道炎症，以及咽炎、扁桃体炎、风湿病和支气管炎。虽然先前的研究表明 BDG 具有抗炎活性，但它对溃疡性结肠炎（UC）的影响尚未得到探索。
研究蝙蝠葛根茎提取物对 2,4,6-三硝基苯磺酸（TNBS）诱导的 UC 模型小鼠肠道的抗炎作用。
采用结肠内给予 TNBS 的方法诱导 UC 模型。BDG（100、200 和 400mg/kg/天）和柳氮磺胺吡啶（500mg/kg/天）连续口服 7 天。通过大体观察、宏观和组织学分析以及髓过氧化物酶（MPO）活性的积累来评估炎症程度。通过酶联免疫吸附试验测定促炎介质肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β和 IL-6 的含量。通过免疫组织化学分析评估环氧化酶（COX）-2 的表达。
不同剂量的 BDG 治疗显著改善了宏观损伤和组织学变化，减少了 MPO 活性的积累，剂量依赖性地降低了血清和结肠组织中 TNF-α、IL-1β和 IL-6 的水平。此外，BDG 给药有效地降低了 COX-2 在结肠中的过度表达。
我们首次证明 BDG 在 TNBS 诱导的小鼠结肠炎中具有显著的肠道抗炎作用，这可能与下调促炎介质的产生和表达有关，表明它可能对治疗炎症性肠病有益。
