Biomedicine Key Laboratory of Shaanxi Province, Northwest University, No. 229 Taibai North Road, Xi'an 710069, People's Republic of China.
J Ethnopharmacol. 2011 Oct 31;138(1):53-9. doi: 10.1016/j.jep.2011.08.019. Epub 2011 Aug 19.
Pulvis Fellis Suis is used in folk medicines to treat intestinal diseases, acute pharyngitis, whooping cough and asthma in China. Although several reports indicate that Pulvis Fellis Suis display diverse biological activities, such as antibacterial, anti-inflammatory and anti-infusorian effects, its effects on ulcerative colitis have not been previously explored.
The purpose of the present study is to assess the anti-inflammatory effect of Pulvis Fellis Suis (PFS) extract in acute ulcerative colitis model induced by trinitrobenzene sulfonic acid (TNBS) in mice.
Different doses of Pulvis Fellis Suis extract (100, 200 and 400mg/kg/day) and sulfasalazine (500mg/kg/day) were administered by gavage for 7days after the induction of colitis with TNBS. The efficacy of PFS was studied by macroscopical and histological scoring systems as well as myeloperoxidase (MPO) activity. Serum levels, including tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were assayed by enzyme-linked immunoassay. The expression of cyclooxygenase (COX)-2 in the colons was assessed by immunohistochemical analysis.
Treatment with PFS significantly attenuated macroscopic damage as compared with TNBS (P<0.01). Histological analysis showed that PFS improved the microscopic structure and preserved some areas of the colonic mucosa structure. In addition, administration of PFS effectively inhibited COX-2 protein expression and MPO activity accumulation. TNF-α and IL-6 levels were also diminished dose-dependently (P<0.05, P<0.01), and IL-6 level obtained had no significant results by small dose of PFS. All the effects of these parameters were comparable to that of the standard sulfasalazine, especially at the highest dose level.
We have shown for the first time that PFS has an anti-inflammatory effect in TNBS-induced ulcerative colitis which might be related to the reduction of up-regulated TNF-α and IL-6 production, and that it may have therapeutic value in the setting of inflammatory bowel disease (IBD).
在中国，猪胆粉被民间医学用于治疗肠道疾病、急性咽炎、百日咳和哮喘。尽管有几项报告表明猪胆粉具有多种生物活性，如抗菌、抗炎和抗纤毛虫作用，但它对溃疡性结肠炎的作用尚未得到先前的探索。
本研究的目的是评估猪胆粉（PFS）提取物在三硝基苯磺酸（TNBS）诱导的小鼠急性溃疡性结肠炎模型中的抗炎作用。
用 TNBS 诱导结肠炎后，通过灌胃给予不同剂量的猪胆粉提取物（100、200 和 400mg/kg/天）和柳氮磺胺吡啶（500mg/kg/天），共 7 天。通过宏观和组织学评分系统以及髓过氧化物酶（MPO）活性研究 PFS 的疗效。通过酶联免疫吸附试验测定血清水平，包括肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）。通过免疫组织化学分析评估结肠中环氧化酶（COX）-2的表达。
与 TNBS 相比，PFS 治疗显著减轻了宏观损伤（P<0.01）。组织学分析表明，PFS 改善了微观结构并保留了结肠黏膜结构的一些区域。此外，PFS 给药有效抑制了 COX-2 蛋白表达和 MPO 活性积累。TNF-α和 IL-6 水平也呈剂量依赖性降低（P<0.05，P<0.01），并且 PFS 的小剂量对 IL-6 水平没有显著影响。所有这些参数的作用都与标准柳氮磺胺吡啶相当，尤其是在最高剂量水平。
我们首次表明，PFS 对 TNBS 诱导的溃疡性结肠炎具有抗炎作用，这可能与下调 TNF-α和 IL-6 产生有关，并且它在炎症性肠病（IBD）的治疗中可能具有治疗价值。
