Low Dimensional Materials Research Centre (LDMRC), Department of Physics, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Mushroom Research Centre, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Sci Rep. 2016 Jul 20;6:29879. doi: 10.1038/srep29879.
The discovery of semiconducting behavior of deoxyribonucleic acid (DNA) has resulted in a large number of literatures in the study of DNA electronics. Sequence-specific electronic response provides a platform towards understanding charge transfer mechanism and therefore the electronic properties of DNA. It is possible to utilize these characteristic properties to identify/detect DNA. In this current work, we demonstrate a novel method of DNA-based identification of basidiomycetes using current-voltage (I-V) profiles obtained from DNA-specific Schottky barrier diodes. Electronic properties such as ideality factor, barrier height, shunt resistance, series resistance, turn-on voltage, knee-voltage, breakdown voltage and breakdown current were calculated and used to quantify the identification process as compared to morphological and molecular characterization techniques. The use of these techniques is necessary in order to study biodiversity, but sometimes it can be misleading and unreliable and is not sufficiently useful for the identification of fungi genera. Many of these methods have failed when it comes to identification of closely related species of certain genus like Pleurotus. Our electronics profiles, both in the negative and positive bias regions were however found to be highly characteristic according to the base-pair sequences. We believe that this simple, low-cost and practical method could be useful towards identifying and detecting DNA in biotechnology and pathology.
脱氧核糖核酸（DNA）半导体行为的发现导致了大量关于 DNA 电子学的文献研究。序列特异性电子响应为理解电荷转移机制和因此 DNA 的电子性质提供了一个平台。可以利用这些特征性质来识别/检测 DNA。在本研究中，我们使用 DNA 特异性肖特基势垒二极管获得的电流-电压（I-V）曲线，展示了一种基于 DNA 的鉴定担子菌的新方法。计算了理想因子、势垒高度、分流电阻、串联电阻、开启电压、膝电压、击穿电压和击穿电流等电子特性，并将其与形态学和分子特征技术进行比较，用于量化鉴定过程。为了研究生物多样性，这些技术的使用是必要的，但有时它可能会产生误导和不可靠，并且对于鉴定真菌属的物种并不足够有用。在某些属的某些密切相关的物种的鉴定方面，许多这些方法都失败了，例如，侧耳属。然而，我们发现，根据碱基对序列，我们的电子曲线在负偏压和正偏压区域都具有高度的特征性。我们相信，这种简单、低成本和实用的方法可能有助于生物技术和病理学中的 DNA 识别和检测。
