Prasad Uday Uma Shankar, Bandyopadhyay Tarun Kanti, Bhunia Biswanath
a Department of Chemical Engineering , National Institute of Technology , Agartala , India.
b Department of Bio Engineering , National Institute of Technology , Agartala , India.
Bioengineered. 2016 Nov;7(6):424-431. doi: 10.1080/21655979.2016.1180486. Epub 2016 Jul 19.
The present investigation is mainly concerned with the rapid development of extracellular xylanase assay conditions by using Taguchi methodology. The extracellular xylanase was produced from Aspergillus niger (KP874102.1), a new strain isolated from a soil sample of the Baramura forest, Tripura West, India. Four physical parameters including temperature, pH, buffer concentration and incubation time were considered as key factors for xylanase activity and were optimized using Taguchi robust design methodology for enhanced xylanase activity. The main effect, interaction effects and optimal levels of the process factors were determined using signal-to-noise (S/N) ratio. The Taguchi method recommends the use of S/N ratio to measure quality characteristics. Based on analysis of the S/N ratio, optimal levels of the process factors were determined. Analysis of variance (ANOVA) was performed to evaluate statistically significant process factors. ANOVA results showed that temperature contributed the maximum impact (62.58%) on xylanase activity, followed by pH (22.69%), buffer concentration (9.55%) and incubation time (5.16%). Predicted results showed that enhanced xylanase activity (81.47%) can be achieved with pH 2, temperature 50°C, buffer concentration 50 Mm and incubation time 10 min.
本研究主要关注利用田口方法快速开发胞外木聚糖酶的测定条件。胞外木聚糖酶由黑曲霉（KP874102.1）产生，该菌株是从印度西特里普拉巴拉穆拉森林的土壤样本中分离出的新菌株。温度、pH值、缓冲液浓度和孵育时间这四个物理参数被视为影响木聚糖酶活性的关键因素，并采用田口稳健设计方法进行优化以提高木聚糖酶活性。使用信噪比（S/N）确定工艺因素的主要效应、交互效应和最佳水平。田口方法建议使用信噪比来衡量质量特性。基于信噪比分析，确定了工艺因素的最佳水平。进行方差分析（ANOVA）以评估具有统计学意义的工艺因素。方差分析结果表明，温度对木聚糖酶活性的影响最大（62.58%），其次是pH值（22.69%）、缓冲液浓度（9.55%）和孵育时间（5.16%）。预测结果表明，在pH值为2、温度为50°C、缓冲液浓度为50 mM和孵育时间为10分钟的条件下，木聚糖酶活性可提高81.47%。
