Department of Chemical Engineering, National Institute of Technology Agartala, Agartala, 799046, India.
Department of Chemical Engineering, Birla Institute of Technology Mesra, Ranchi, Jharkhand, 835215, India.
Appl Biochem Biotechnol. 2022 Nov;194(11):5403-5418. doi: 10.1007/s12010-022-04053-w. Epub 2022 Jul 2.
Prodigiosin is natural red colourant derived from Serratia marcescens. However, the high cost of prodigiosin restricts its use in food and pharmaceutical industries, which can be addressed with the design of a suitable extraction procedure. Therefore, the present study aims to use Taguchi methodology to optimize various process parameters during ultrasound-assisted extraction (UAE) to get a higher prodigiosin extraction yield. The most significant contribution comes from the solid-to-liquid ratio (36.66%), followed by sonication of duty cycle (34.82%), medium pH (15.7%), and acoustic intensity (12.82%). The Taguchi technique predicts the highest optimal yield using the solid-liquid ratio (0.3 g/mL), duty cycle sonication (75%), acoustic intensity (12.5 w/cm), and medium pH (3) as parameters. When the extraction conditions were optimized, the yield of prodigiosin increased by 4166.89 mg/L. In the future, the above extraction conditions determined using Taguchi approach will be applied for large-scale extraction of prodigiosin. Finally, a second-order kinetic model is used to suit the batch extraction investigation and the second-order rate constant (k) has a value of 4 × 10 L/mg/min. In the future, the rate constant, which is reported for the first time, will be used to create a batch extractor for commercial extraction of prodigiosin. Prodigiosin has also been shown to have substantial antioxidant and scavenging properties, which increase in a dose-dependent way with prodigiosin concentration. Because of its antioxidant and scavenging properties, prodigiosin can be used as food additives or pharmaceutical ingredients in industries.
灵菌红素是一种天然红色着色剂，来源于粘质沙雷氏菌。然而，灵菌红素的高成本限制了它在食品和制药行业的应用，可以通过设计合适的提取工艺来解决。因此，本研究旨在使用田口方法优化超声辅助提取（UAE）过程中的各种工艺参数，以获得更高的灵菌红素提取产率。最重要的贡献来自固液比（36.66%），其次是占空比超声（34.82%）、介质 pH（15.7%）和声强（12.82%）。田口技术预测使用固液比（0.3 g/mL）、占空比超声（75%）、声强（12.5 w/cm）和介质 pH（3）作为参数时，最高产率最优。当优化提取条件时，灵菌红素的产率增加了 4166.89 mg/L。在未来，将采用田口方法确定的上述提取条件进行灵菌红素的大规模提取。最后，使用二阶动力学模型拟合批处理提取研究，二阶速率常数（k）的值为 4×10 L/mg/min。未来，将首次使用该速率常数为商业提取灵菌红素创建一个批量提取器。灵菌红素还具有显著的抗氧化和清除自由基特性，其抗氧化和清除自由基的特性随灵菌红素浓度呈剂量依赖性增加。由于其抗氧化和清除自由基的特性，灵菌红素可作为食品添加剂或制药成分在工业中使用。
