Ojeda Edilberto, Agirre Mireia, Villate-Beitia Ilia, Mashal Mohamed, Puras Gustavo, Zarate Jon, Pedraz Jose L
NanoBioCel Group, University of the Basque Country (UPV-EHU), Vitoria-Gasteiz, Spain.
Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, Spain.
Methods Mol Biol. 2016;1445:63-75. doi: 10.1007/978-1-4939-3718-9_5.
Niosome formulations for gene delivery purposes are based on nonionic surfactants, helper lipids, and cationic lipids that interact electrostatically with negatively charged DNA molecules to form the so-called nioplexes. Niosomes are elaborated by different techniques, such as solvent emulsion-evaporation, thin film hydration, hand-shaking, dissolvent injection, and microfluidization method, among many others. In this chapter, we have described some protocols for the elaboration of niosomes and nioplexes and their physicochemical characterization that guarantees the quality criteria of the formulation in terms of size, morphology, ζ-potential, and stability.
用于基因递送目的的脂质体配方基于非离子表面活性剂、辅助脂质和阳离子脂质，它们与带负电荷的DNA分子发生静电相互作用，形成所谓的脂质复合物。脂质体可通过不同技术制备，如溶剂乳化蒸发法、薄膜水化法、手摇法、溶剂注入法和微流化法等。在本章中，我们描述了一些脂质体和脂质复合物的制备方案及其物理化学特性，这些特性保证了制剂在尺寸、形态、ζ电位和稳定性方面的质量标准。
