Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, PR China; Department of Chemistry and Biochemistry, North Dakota State University, Fargo, ND 58105, United States.
Research Center for Bioengineering and Sensing Technology, University of Science & Technology Beijing, Beijing 100083, PR China.
Anal Chim Acta. 2017 Nov 15;993:79-86. doi: 10.1016/j.aca.2017.09.025. Epub 2017 Sep 27.
The authors describe a magnetized carbon nanotube (MCNT)-based lateral flow strip biosensor for visual detection of proteins directly in whole blood avoiding complex purification and sample pre-treatments. MCNT were synthesized by coating FeO nanoparticles on the shortened multiwalled carbon nanotube (CNT) surface via co-precipitation of ferric and ferrous ions within a dispersion of shorten multiwalled CNTs. The antibody-modified MCNTs were used to capture target protein in whole blood; the formed MCNT-antibody-target protein complexes were applied to the lateral flow strip biosensor, in which a capture antibody was immobilized on the test zone of the biosensor. The captured MCNTs on the test zone and control zone were producing characteristic brown/black bands, and this enabled target protein to be visually detected. Quantification was accomplished by reading the intensities of the bands with a portable strip reader. Rabbit IgG was used as a model target to demonstrate the proof-of-concept. After systematic optimizations of assay parameters, the detection limit of the assay in whole blood was determined to be 10 ng mL (S/N = 3) with a linear dynamic range of 10-200 ng mL. This study provides a rapid and low-cost approach for detecting proteins in blood, showing great promise for clinical application and biomedical diagnosis, particularly in limited resource settings.
作者描述了一种基于磁性碳纳米管（MCNT）的侧向流动条生物传感器，用于直接在全血中可视化检测蛋白质，避免了复杂的纯化和样品预处理。MCNT 通过在短切多壁碳纳米管（CNT）分散体中共同沉淀铁离子和亚铁离子，在缩短的多壁 CNT 表面上涂覆 FeO 纳米颗粒来合成。抗体修饰的 MCNT 用于捕获全血中的靶蛋白;形成的 MCNT-抗体-靶蛋白复合物被应用于侧向流动条生物传感器，其中捕获抗体固定在生物传感器的测试区。测试区和对照区上捕获的 MCNT 产生特征性的棕色/黑色条带，从而可以目视检测靶蛋白。通过用便携式条带读取器读取条带的强度来完成定量。兔 IgG 被用作模型靶标来证明概念验证。在对测定参数进行系统优化后，该测定法在全血中的检测限确定为 10ng mL（S/N=3），线性动态范围为 10-200ng mL。该研究提供了一种快速且低成本的方法来检测血液中的蛋白质，有望在临床应用和生物医学诊断中得到应用，特别是在资源有限的环境中。
