Yaman Turan, Yener Zabit, Celik Ismail
Department of Pathology, Faculty of Veterinary Medicine, Yuzuncu Yil University, Van, Turkey.
Department of Biology, Faculty of Sciences and Letters, Yuzuncu Yil University, Van, Turkey.
BMC Complement Altern Med. 2016 Jul 21;16:232. doi: 10.1186/s12906-016-1217-7.
Natural honey (honey) is considered as a part of traditional medicine all over the world. It has both antimicrobial and antioxidant properties, useful in stimulation of wounds and burns healing and gastric ulcers treatment. The aim of this study, for the first time, was to investigate the antioxidant properties and protective role of honey against carcinogen chemical aflatoxin (AF) exposure in rats, which were evaluated by histopathological changes in liver and kidney, measuring level of serum marker enzymes [aspartate aminotransferase (AST), alanin aminotransferase (ALT), gamma glutamil transpeptidase (GGT)], antioxidant defense systems [Reduced glutathione (GSH), glutathione reductase (GR), superoxide dismutase (SOD), glutathione-S-transferase (GST) and catalase (CAT)], and lipid peroxidation content in liver, erythrocyte, brain, kidney, heart and lungs.
Eighteen healthy Sprague-Dawley rats were randomly allocated into three experimental groups: A (Control), B (AF-treated) and C (AF + honey-treated). While rats in group A were fed with a diet without AF, B, and C groups received 25 μg of AF/rat/day, where C group additionally received 1 mL/kg of honey by gavage for 90 days.
At the end of the 90-day experimental period, we found that the honey supplementation decreased the lipid peroxidation and the levels of enzyme associated with liver damage, increased enzymatic and non-enzymatic antioxidants in the AF + honey-treated rats. Hepatoprotective and nephroprotective effects of honey is further substantiated by showing almost normal histological architecture in AF + honey-treated group, compared to degenerative changes in the liver and kidney of AF-treated rats. Additionally, honey supplementation ameliorated antioxidant defens systems and lipid peroxidation in content in other tissues of AF + honey treated rats.
The present study indicates that honey has a hepatoprotective and nephroprotective effect in rats with experimental aflatoxicosis due to its antioxidant activity.
天然蜂蜜在世界各地都被视为传统医学的一部分。它具有抗菌和抗氧化特性，有助于促进伤口和烧伤愈合以及治疗胃溃疡。本研究首次旨在通过观察大鼠肝脏和肾脏的组织病理学变化、测量血清标志物酶[天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、γ-谷氨酰转肽酶（GGT）]水平、抗氧化防御系统[还原型谷胱甘肽（GSH）、谷胱甘肽还原酶（GR）、超氧化物歧化酶（SOD）、谷胱甘肽-S-转移酶（GST）和过氧化氢酶（CAT）]以及肝脏、红细胞、脑、肾脏、心脏和肺中的脂质过氧化含量，来研究蜂蜜对大鼠接触致癌化学物质黄曲霉毒素（AF）的抗氧化特性和保护作用。
将18只健康的Sprague-Dawley大鼠随机分为三个实验组：A组（对照组）、B组（AF处理组）和C组（AF+蜂蜜处理组）。A组大鼠喂食不含AF的饲料，B组和C组大鼠每天每只接受25μg的AF，其中C组另外通过灌胃给予1mL/kg的蜂蜜，持续90天。
在90天的实验期结束时，我们发现补充蜂蜜可降低脂质过氧化和与肝损伤相关的酶水平，增加AF+蜂蜜处理组大鼠的酶促和非酶促抗氧化剂。与AF处理组大鼠肝脏和肾脏的退行性变化相比，AF+蜂蜜处理组几乎正常的组织结构进一步证实了蜂蜜的肝脏保护和肾脏保护作用。此外，补充蜂蜜改善了AF+蜂蜜处理组大鼠其他组织中的抗氧化防御系统和脂质过氧化含量。
本研究表明，蜂蜜因其抗氧化活性对实验性黄曲霉毒素中毒的大鼠具有肝脏保护和肾脏保护作用。
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