Ali Fatma Abo Zakaib, Abdel-Maksoud Fatma M, Abd Elaziz Hekmat Osman, Al-Brakati Ashraf, Elmahallawy Ehab Kotb
Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Sohag University, Sohag 82524, Egypt.
Department of Anatomy and Histology, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, Egypt.
Animals (Basel). 2021 Feb 16;11(2):509. doi: 10.3390/ani11020509.
Liver sinusoids are lined by fenestrated endothelial cells surrounded by perisinusoidal cells, Kupffer cells, and pit cells, as well as large granular lymphocytes. The functional ability of the liver cells can be substantially modified by exposure to toxins. In the current work, we assessed the histopathological and ultrastructural effects of a time-course exposure to aflatoxin B1 (AFB1) on the hepatic structures of rats. A total of 30 adult female Wistar rats were randomly divided into three groups: a control group, a group orally administered 250 µg/kg body weight/day of AFB1 for 5 days/week over 4 weeks, and a group that received the same AFB1 treatment but over 8 weeks. Histopathological and ultrastructural examinations of hepatocytes revealed massive vacuolar degeneration and signs of necrosis. Furthermore, the rat liver of the treated group exhibited damage to the sinusoidal endothelium, invasion of the space of Disse with hyperactive Kupffer cells, and some immune cells, as well as Ito cells overloaded with lipids. In addition, damaged telocytes were observed. Taken together, our results indicate that AFB1 induces irreversible adverse effects on the livers of rats.
肝血窦由有窗孔的内皮细胞排列而成，周围有窦周细胞、库普弗细胞、陷窝细胞以及大颗粒淋巴细胞。肝细胞的功能能力会因接触毒素而发生显著改变。在当前研究中，我们评估了黄曲霉毒素B1（AFB1）随时间变化的暴露对大鼠肝脏结构的组织病理学和超微结构影响。总共30只成年雌性Wistar大鼠被随机分为三组：对照组、一组以250 µg/kg体重/天的剂量口服AFB1，每周5天，持续4周；另一组接受相同的AFB1处理，但持续8周。对肝细胞的组织病理学和超微结构检查显示大量空泡变性和坏死迹象。此外，处理组大鼠肝脏的窦状内皮受损，狄氏间隙有库普弗细胞和一些免疫细胞活跃浸润，以及脂滴过载的贮脂细胞。另外，还观察到受损的间充质干细胞。综上所述，我们的结果表明AFB1对大鼠肝脏诱导产生了不可逆的不良影响。
