Ibrahim Abdelazim, Eldaim Mabrouk A Abd, Abdel-Daim Mohamed M
Pathology Department, College of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.
Department of Pathology, College of Veterinary Medicine and Animal Resources, King Faisal University, Al-Ahsa, Saudi Arabia.
Cytotechnology. 2016 Aug;68(4):1039-48. doi: 10.1007/s10616-015-9860-2. Epub 2015 Feb 27.
Cisplatin is one of the most potent and effective chemotherapeutic agents. However, its antineoplastic use is limited due to its cumulative nephrotoxic side effects. Therefore, the present study was undertaken to examine the nephroprotective potential of dietary bee honey and royal jelly against subchronic cisplatin toxicity in rats. Male Wistar rats were randomly divided into controls, cisplatin-treated, bee honey-pretreated cisplatin-treated and royal jelly-pretreated cisplatin-treated groups. Bee honey and royal jelly were given orally at doses of 20 and 100 mg/kg, respectively. Subchronic toxicity was induced by cisplatin (1 mg/kg bw, ip), twice weekly for 10 weeks. Cisplatin treated animals revealed a significant increase in serum level of renal injury products (urea, creatinine and uric acid). Histopathologically, cisplatin produced pronounced tubulointerstitial injuries, upregulated the fibrogenic factors, α-smooth muscle actin (α-SMA) and transforming growth factor β1(TGF-β1), and downregulated the cell proliferation marker, bromodeoxyuridine (Brdu). Dietary bee honey and royal jelly normalized the elevated serum renal injury product biomarkers, improved the histopathologic changes, reduced the expression of α-SMA and TGF-β1 and increased the expression of Brdu. Therefore, it could be concluded that bee honey, and royal jelly could be used as dietary preventive natural products against subchronic cisplatin-induced renal injury.
顺铂是最有效的化疗药物之一。然而，由于其累积性肾毒性副作用，其抗肿瘤应用受到限制。因此，本研究旨在探讨食用蜂蜜和蜂王浆对大鼠亚慢性顺铂毒性的肾保护潜力。雄性Wistar大鼠被随机分为对照组、顺铂治疗组、蜂蜜预处理顺铂治疗组和蜂王浆预处理顺铂治疗组。蜂蜜和蜂王浆分别以20和100mg/kg的剂量口服给药。顺铂（1mg/kg体重，腹腔注射）诱导亚慢性毒性，每周两次，共10周。顺铂治疗的动物血清中肾损伤产物（尿素、肌酐和尿酸）水平显著升高。组织病理学上，顺铂导致明显的肾小管间质损伤，上调纤维化因子α-平滑肌肌动蛋白（α-SMA）和转化生长因子β1（TGF-β1），并下调细胞增殖标志物溴脱氧尿苷（Brdu）。食用蜂蜜和蜂王浆可使升高的血清肾损伤产物生物标志物恢复正常，改善组织病理学变化，降低α-SMA和TGF-β1的表达，并增加Brdu的表达。因此，可以得出结论，蜂蜜和蜂王浆可作为预防亚慢性顺铂诱导肾损伤的食用天然产物。
