Meeks-Wagner D R, Dennis E S, Tran Thanh Van K, Peacock W J
Division of Plant Industry, Commonwealth Scientific and Industrial Research Organization, Canberra, ACT, Australia.
Plant Cell. 1989 Jan;1(1):25-35. doi: 10.1105/tpc.1.1.25.
Since the transition from vegetative to floral development in plants is likely to be influenced by gene expression in several plant organs, we have used an in vitro system, the tobacco "thin cell layer" system as a model for investigating gene expression associated with the initiation of flowering in higher plants. cDNA cloning has been used to identify mRNAs abundant during thin cell layer floral initiation. These genes are expressed in thin cell layer explants initiating floral meristems but not in thin cell layer explants initiating vegetative shoot meristems or possessing roots. Two of these genes are expressed transcriptionally in incipient floral apices during normal plant development. Transcripts of these genes, plus a third gene, occur at low levels in several plant organs and at high levels in the roots, with the maximum levels of root expression reached just prior to the formation of floral meristems.
由于植物从营养生长向生殖生长的转变可能受到多个植物器官中基因表达的影响，我们使用了一种体外系统——烟草“薄细胞层”系统，作为研究高等植物开花起始相关基因表达的模型。cDNA克隆已被用于鉴定薄细胞层花芽起始过程中丰富的mRNA。这些基因在起始花分生组织的薄细胞层外植体中表达，但在起始营养芽分生组织或具有根的薄细胞层外植体中不表达。在正常植物发育过程中，其中两个基因在初期花芽顶端进行转录表达。这些基因的转录本，加上第三个基因，在几个植物器官中低水平表达，在根中高水平表达，在花分生组织形成之前根中的表达水平达到最高。
