Gentili Stefano, Mortali Claudia, Mastrobattista Luisa, Berretta Paolo, Zaami Simona
Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy.
Unit of Forensic Toxicology (UoFT), Department of Anatomical, Histological, Forensic and Orthopedic Sciences, Sapienza University of Rome, Viale Regina Elena 336, (00185) Rome, Italy.
J Pharm Biomed Anal. 2016 Sep 10;129:282-287. doi: 10.1016/j.jpba.2016.07.018. Epub 2016 Jul 14.
A procedure based on headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography/mass spectrometry (GC/MS) has been developed for the determination of most commonly used drugs of abuse in sweat of drivers stopped during roadside controls. DrugWipe 5A sweat screening device was used to collect sweat by a specific pad rubbed gently over forehead skin surface. The procedure involved an acid hydrolysis, a HS-SPME extraction for drugs of abuse but Δ(9)-tetrahydrocannabinol, which was directly extracted in alkaline medium HS-SPME conditions, a GC separation of analytes by a capillary column and MS detection by electron impact ionisation. The method was linear from the limit of quantification (LOQ) to 50ng drug per pad (r(2)≥0.99), with an intra- and inter-assay precision and accuracy always less than 15% and an analytical recovery between 95.1% and 102.8%, depending on the considered analyte. Using the validated method, sweat from 60 apparently intoxicated drivers were found positive to one or more drugs of abuse, showing sweat patches testing as a viable economic and simple alternative to conventional (blood and/or urine) and non conventional (oral fluid) testing of drugs of abuse in drugged drivers.
一种基于顶空固相微萃取（HS-SPME）结合气相色谱/质谱联用（GC/MS）的方法已被开发出来，用于测定在路边检查中被拦下的驾驶员汗液中最常用的滥用药物。DrugWipe 5A汗液筛查装置用于通过在额头皮肤表面轻轻擦拭特定垫子来收集汗液。该方法包括酸水解、对滥用药物进行HS-SPME萃取，但Δ(9)-四氢大麻酚在碱性介质HS-SPME条件下直接萃取，通过毛细管柱对分析物进行GC分离以及通过电子轰击电离进行MS检测。该方法在定量限（LOQ）至每片垫子50 ng药物范围内呈线性（r(2)≥0.99），批内和批间精密度及准确度始终小于15%，分析回收率在95.1%至102.8%之间，具体取决于所考虑的分析物。使用经过验证的方法，发现60名明显醉酒驾驶员的汗液对一种或多种滥用药物呈阳性，表明汗液贴片检测是对吸毒驾驶员进行传统（血液和/或尿液）及非传统（口腔液）滥用药物检测的一种可行的经济且简便的替代方法。
