Astrid Lindgren Children's Hospital, Karolinska University Hospital, Stockholm, Sweden.
Department of Women's and Children's Health, Karolinska Institutet, Stockholm, Sweden.
Allergy. 2016 Dec;71(12):1728-1735. doi: 10.1111/all.12991. Epub 2016 Aug 22.
Some children with rhinovirus (RV) infections wheeze, but it is unknown whether this is due to more virulent strains of virus or differences in host immune responses. The aim of this study was to investigate the RV species-specific antibody responses measured at a follow-up visit in preschool children in relation to reported time with respiratory symptoms and the presence of different RV species during an acute episode of wheeze.
Nasopharyngeal swabs and blood samples were taken among 120 preschool children (<4 years of age) at an acute episode of wheeze and at a follow-up visit (median 11 weeks later). Nested PCR was used to detect different RV strains, and serum levels of IgG against purified recombinant VP1 proteins from representatives of the three RV species (RV-A, RV-B, and RV-C) were measured by ELISA.
Rhinovirus was detected in 74% (n = 80/108) of the children at the acute visit, and RV-C was the most common subtype (n = 59/80, 74%). An increase in RV-specific IgG was seen in 61% (n = 73) of the children at follow-up, most frequently against RV-A (n = 61/73, 86%) irrespective of the RV strains detected by PCR. Increases in RV-specific IgG against RV-A or against RV-A and RV-C were significantly associated with more respiratory symptoms (p = 0.03, p = 0.007).
Antibody response to recombinant RV VP1 proteins was associated with longer time with respiratory symptoms.
一些感染鼻病毒（RV）的儿童会出现喘息，但尚不清楚这是由于病毒株毒力更强，还是宿主免疫反应的差异所致。本研究旨在调查在学龄前喘息急性发作时随访中测量的 RV 种特异性抗体反应与报告的呼吸道症状持续时间以及不同 RV 种在喘息急性发作时的存在情况之间的关系。
120 名学龄前儿童（<4 岁）在喘息急性发作时和随访时（中位数为 11 周后）采集鼻咽拭子和血样。采用巢式 PCR 检测不同的 RV 株，采用 ELISA 法检测针对三种 RV 种（RV-A、RV-B 和 RV-C）的代表纯化重组 VP1 蛋白的血清 IgG 水平。
在急性就诊时，74%（n = 80/108）的儿童中检测到鼻病毒，RV-C 是最常见的亚型（n = 59/80，74%）。在随访时，61%（n = 73）的儿童出现 RV 特异性 IgG 增加，最常见的是针对 RV-A（n = 61/73，86%），而与通过 PCR 检测到的 RV 株无关。针对 RV-A 或针对 RV-A 和 RV-C 的 RV 特异性 IgG 增加与呼吸道症状持续时间更长显著相关（p = 0.03，p = 0.007）。
针对重组 RV VP1 蛋白的抗体反应与呼吸道症状持续时间较长有关。
