Kushwaha Hemant R, Joshi Rohit, Pareek Ashwani, Singla-Pareek Sneh L
Microbial Engineering, International Centre for Genetic Engineering and Biotechnology New Delhi, India.
Plant Stress Biology, International Centre for Genetic Engineering and Biotechnology New Delhi, India.
Front Plant Sci. 2016 Jun 28;7:923. doi: 10.3389/fpls.2016.00923. eCollection 2016.
Response to stress represents a highly complex mechanism in plants involving a plethora of genes and gene families. It has been established that plants use some common set of genes and gene families for both biotic and abiotic stress responses leading to cross-talk phenomena. One such family, Meprin And TRAF Homology (MATH) domain containing protein (MDCP), has been known to be involved in biotic stress response. In this study, we present genome-wide identification of various members of MDCP family from both Arabidopsis and rice. A large number of members identified in Arabidopsis and rice indicate toward an expansion and diversification of MDCP family in both the species. Chromosomal localization of MDCP genes in Arabidopsis and rice reveals their presence in a few specific clusters on various chromosomes such as, chromosome III in Arabidopsis and chromosome X in rice. For the functional analysis of MDCP genes, we used information from publicly available data for plant growth and development as well as biotic stresses and found differential expression of various members of the family. Further, we narrowed down 11 potential candidate genes in rice which showed high expression in various tissues and development stages as well as biotic stress conditions. The expression analysis of these 11 genes in rice using qRT-PCR under drought and salinity stress identified OsM4 and OsMB11 to be highly expressed in both the stress conditions. Taken together, our data indicates that OsM4 and OsMB11 can be used as potential candidates for generating stress resilient crops.
植物对胁迫的响应是一个高度复杂的机制，涉及大量基因和基因家族。已经确定，植物在生物胁迫和非生物胁迫响应中使用一些共同的基因和基因家族，从而导致相互作用现象。其中一个家族，即含有Meprin和TRAF同源结构域（MATH）的蛋白质（MDCP），已知参与生物胁迫响应。在本研究中，我们对拟南芥和水稻中MDCP家族的各个成员进行了全基因组鉴定。在拟南芥和水稻中鉴定出的大量成员表明该家族在这两个物种中都有扩展和多样化。MDCP基因在拟南芥和水稻中的染色体定位显示它们存在于不同染色体上的一些特定簇中，例如拟南芥的第三条染色体和水稻的第十条染色体。为了对MDCP基因进行功能分析，我们使用了公开可用的有关植物生长发育以及生物胁迫的数据信息，发现该家族各个成员存在差异表达。此外，我们在水稻中筛选出11个潜在的候选基因，这些基因在不同组织、发育阶段以及生物胁迫条件下均高表达。利用qRT-PCR对水稻中的这11个基因在干旱和盐胁迫下进行表达分析，确定OsM4和OsMB11在这两种胁迫条件下均高表达。综上所述，我们的数据表明OsM4和OsMB11可作为培育抗逆作物的潜在候选基因。
