van Waarde A, Lombarts A, van den Thillart G, Erkelens C, Lugtenburg J
Department of Animal Physiology, Gorlaeus Laboratories, University of Leiden, The Netherlands.
Haematologia (Budap). 1989;22(2):79-87.
We used 31P Nuclear Magnetic Resonance (NMR) spectroscopy to investigate whether regeneration of ATP in outdated human blood is accompanied by Mg-ATP recomplexation. We found that the loss of 'total ATP' (i.e. the sum of ATP4- + MG-ATP2-) which occurs during blood storage is completely reversible by treatment of RBC with precursors for purine nucleotide salvage. The magnesium-ATP complex, however, is only partially (70%) recovered due to a continuous decline of cytosolic free Mg2+ with increasing RBC age. Total RBC magnesium, as measured by flame absorption spectroscopy, did not show any significant change under these conditions.
我们使用31P核磁共振（NMR）光谱来研究过期人血中ATP的再生是否伴随着Mg-ATP的重新络合。我们发现，在血液储存期间发生的“总ATP”（即ATP4- + Mg-ATP2-的总和）的损失，通过用嘌呤核苷酸补救的前体处理红细胞可完全逆转。然而，由于随着红细胞年龄的增加，胞质游离Mg2+持续下降，镁-ATP复合物仅部分（70%）恢复。通过火焰吸收光谱法测量的总红细胞镁在这些条件下没有显示出任何显著变化。
