Bjornson Marta, Song Xingshun, Dandekar Abhaya, Franz Annaliese, Drakakaki Georgia, Dehesh Katayoon
Department of Plant Sciences, University of California, Davis, USA; Department of Plant Sciences, University of California, Davis, USA.
College of Life Science, Northeast Forestry University, Harbin, China.
Bio Protoc. 2015 Jul 5;5(13). doi: 10.21769/bioprotoc.1519.
Unbiased screening approaches are powerful tools enabling identification of novel players in biological processes. Chemical genetic screening refers to the technique of using a reporter response, such as expression of luciferase driven by a promoter of interest, to discover small molecules that affect a given process when applied to plants. These chemicals then act as tools for identification of regulatory components that could not otherwise be detected by forward genetic screens due to gene family redundancy or mutant lethality. This protocol describes a chemical genetic screen using seedlings, which has led to recognition of novel players in the plant general stress response.
无偏筛选方法是强大的工具，能够识别生物过程中的新参与者。化学遗传学筛选是指利用报告基因反应（如由感兴趣的启动子驱动的荧光素酶表达）来发现小分子的技术，这些小分子应用于植物时会影响特定过程。由于基因家族冗余或突变致死性，正向遗传学筛选无法检测到这些调控成分，而这些化学物质随后可作为识别这些调控成分的工具。本方案描述了一种使用幼苗进行的化学遗传学筛选，该筛选已促成了对植物一般胁迫反应中新参与者的识别。
