Department of Chemistry, Faculty of Food Sciences, Warsaw University of Life Sciences (WULS-SGGW), Nowoursynowska 159C St., 02-776 Warsaw, Poland.
Department of Chemistry, Faculty of Food Sciences, Warsaw University of Life Sciences (WULS-SGGW), Nowoursynowska 159C St., 02-776 Warsaw, Poland.
Food Chem. 2016 Dec 15;213:450-456. doi: 10.1016/j.foodchem.2016.06.102. Epub 2016 Jun 30.
This study determined and compared the contents of bioactive components in plant seed oils extracted with n-hexane (Soxhlet method) and chloroform/methanol (Folch method) from coriander, caraway, anise, nutmeg and white mustard seeds. Oleic acid dominated among unsaturated fatty acids in nutmeg and anise seed oils while petroselinic acid was present in coriander and caraway oils. Concerning sterols, β-sitosterol was the main component in seed oils extracted with both methods. The content of total phenolics in nutmeg, white mustard and coriander seed oils extracted with chloroform/methanol was higher than in their counterparts prepared with n-hexane. The seed oil samples extracted according to the Folch method exhibited a higher ability to scavenge DPPH radicals compared to the oil samples prepared with the Soxhlet method. DPPH values of the methanolic extracts derived from oils produced with the Folch method were also higher than in the oils extracted with n-hexane.
本研究采用正己烷(索氏提取法)和氯仿/甲醇(Folch 法)从芫荽籽、葛缕子籽、大茴香籽、肉豆蔻和白芥籽油中提取植物种子油,并测定和比较了其中生物活性成分的含量。在肉豆蔻和大茴香籽油的不饱和脂肪酸中,油酸占主导地位,而在芫荽籽和葛缕子籽油中则存在岩芹酸。就甾醇而言,β-谷甾醇是这两种方法提取的种子油中的主要成分。用氯仿/甲醇提取的肉豆蔻、白芥和芫荽籽油中的总酚含量高于用正己烷提取的相应油。与用索氏提取法制备的油相比,根据 Folch 法提取的种子油样品对 DPPH 自由基的清除能力更高。Folch 法提取的油中衍生的甲醇提取物的 DPPH 值也高于用正己烷提取的油。