Mohiti-Asli M, Saha S, Murphy S V, Gracz H, Pourdeyhimi B, Atala A, Loboa E G
Joint Department of Biomedical Engineering at University of North Carolina at Chapel Hill, North Carolina State University, Raleigh, North Carolina, 27695.
Wake Forest Institute for Regenerative Medicine, Wake Forest University School of Medicine, Winston-Salem, North Carolina, 27157.
J Biomed Mater Res B Appl Biomater. 2017 Feb;105(2):327-339. doi: 10.1002/jbm.b.33520. Epub 2015 Oct 28.
This article presents successful incorporation of ibuprofen in polylactic acid (PLA) nanofibers to create scaffolds for the treatment of both acute and chronic wounds. Nanofibrous PLA scaffolds containing 10, 20, or 30 wt % ibuprofen were created and ibuprofen release profiles quantified. In vitro cytotoxicity to human epidermal keratinocytes (HEK) and human dermal fibroblasts (HDF) of the three scaffolds with varying ibuprofen concentrations were evaluated and compared to pure PLA nanofibrous scaffolds. Thereafter, scaffolds loaded with ibuprofen at the concentration that promoted human skin cell viability and proliferation (20 wt %) were evaluated in vivo in nude mice using a full thickness skin incision model to determine the ability of these scaffolds to promote skin regeneration and/or assist with scarless healing. Both acellular and HEK and HDF cell-seeded 20 wt % ibuprofen loaded nanofibrous bandages reduced wound contraction compared with wounds treated with Tegaderm™ and sterile gauze. Newly regenerated skin on wounds treated with cell-seeded 20 wt % ibuprofen bandages exhibited significantly greater blood vessel formation relative to acellular ibuprofen bandages. We have found that degradable anti-inflammatory scaffolds containing 20 wt % ibuprofen promote human skin cell viability and proliferation in vitro, reduce wound contraction in vivo, and when seeded with skin cells, also enhance new blood vessel formation. The approaches and results reported here hold promise for multiple skin tissue engineering and wound healing applications. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 327-339, 2017.
本文介绍了成功地将布洛芬掺入聚乳酸（PLA）纳米纤维中，以制备用于治疗急慢性伤口的支架。制备了含有10%、20%或30%（重量）布洛芬的纳米纤维PLA支架，并对布洛芬的释放曲线进行了量化。评估了三种不同布洛芬浓度的支架对人表皮角质形成细胞（HEK）和人真皮成纤维细胞（HDF）的体外细胞毒性，并与纯PLA纳米纤维支架进行了比较。此后，使用全层皮肤切口模型在裸鼠体内评估了加载有促进人类皮肤细胞活力和增殖浓度（20%，重量）布洛芬的支架，以确定这些支架促进皮肤再生和/或协助无瘢痕愈合的能力。与用德湿可™和无菌纱布治疗的伤口相比，无细胞以及接种了HEK和HDF细胞的20%（重量）布洛芬加载纳米纤维绷带均减少了伤口收缩。与无细胞布洛芬绷带相比，接种了20%（重量）布洛芬绷带治疗的伤口上新再生的皮肤表现出明显更多的血管形成。我们发现，含有20%（重量）布洛芬的可降解抗炎支架在体外可促进人类皮肤细胞活力和增殖，在体内可减少伤口收缩，并且在接种皮肤细胞时还可增强新血管形成。本文报道的方法和结果在多种皮肤组织工程和伤口愈合应用方面具有前景。© 2015威利期刊公司。《生物医学材料研究杂志》B部分：应用生物材料，105B: 327 - 339, 2017。
