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脂肪细胞外基质/基质血管部分凝胶分泌血管生成因子，增强小鼠皮肤伤口愈合。

Adipose Extracellular Matrix/Stromal Vascular Fraction Gel Secretes Angiogenic Factors and Enhances Skin Wound Healing in a Murine Model.

机构信息

Department of Plastic and Cosmetic Surgery, Nanfang Hospital, Southern Medical University, 1838 Guangzhou North Road, Guangzhou, Guangdong 510515, China.

出版信息

Biomed Res Int. 2017;2017:3105780. doi: 10.1155/2017/3105780. Epub 2017 Aug 1.

DOI:10.1155/2017/3105780
PMID:28835892
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5556995/
Abstract

Mesenchymal stem cells are an attractive cell type for cytotherapy in wound healing. The authors recently developed a novel, adipose-tissue-derived, injectable extracellular matrix/stromal vascular fraction gel (ECM/SVF-gel) for stem cell therapy. This study was designed to assess the therapeutic effects of ECM/SVF-gel on wound healing and potential mechanisms. ECM/SVF-gel was prepared for use in nude mouse excisional wound healing model. An SVF cell suspension and phosphate-buffered saline injection served as the control. The expression levels of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and monocyte chemotactic protein-1 (MCP-1) in ECM/SVF-gel were analyzed at different time points. Angiogenesis (tube formation) assays of ECM/SVF-gel extracts were evaluated, and vessels density in skin was determined. The ECM/SVF-gel extract promoted tube formation in vitro and increased the expression of the angiogenic factors VEGF and bFGF compared with those in the control. The expression of the inflammatory chemoattractant MCP-1 was high in ECM/SVF-gel at the early stage and decreased sharply during the late stage of wound healing. The potent angiogenic effects exerted by ECM/SVF-gel may contribute to the improvement of wound healing, and these effects could be related to the enhanced inflammatory response in ECM/SVF-gel during the early stage of wound healing.

摘要

间充质干细胞是一种有吸引力的细胞类型，可用于创伤愈合的细胞治疗。作者最近开发了一种新型的、脂肪组织衍生的、可注射的细胞外基质/基质血管部分凝胶（ECM/SVF-gel）用于干细胞治疗。本研究旨在评估 ECM/SVF-gel 对创伤愈合的治疗效果及其潜在机制。作者制备 ECM/SVF-gel 用于裸鼠切除性创面愈合模型。SVF 细胞混悬液和磷酸盐缓冲盐水注射作为对照。分析 ECM/SVF-gel 中血管内皮生长因子（VEGF）、碱性成纤维细胞生长因子（bFGF）和单核细胞趋化蛋白-1（MCP-1）的表达水平。评估 ECM/SVF-gel 提取物的血管生成（管形成）试验，并确定皮肤中的血管密度。ECM/SVF-gel 提取物在体外促进管形成，并增加了血管生成因子 VEGF 和 bFGF 的表达，与对照组相比。在 ECM/SVF-gel 中，炎症趋化因子 MCP-1 的表达在早期较高，在创伤愈合的晚期急剧下降。ECM/SVF-gel 发挥的强大血管生成作用可能有助于改善创伤愈合，这些作用可能与 ECM/SVF-gel 在创伤愈合早期增强的炎症反应有关。

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