Han YanQuan, Li YuXin, Wang YongZhong, Gao JiaRong, Xia LunZhu, Hong Yan
The First Affiliated Hospital of Anhui University of Chinese Medicine, Grade 3 Laboratory of TCM Preparation, State Administration of Anhui University of Chinese Medicine, Hefei, Anhui 230000, China.
Anhui University of Chinese Medicine, Hefei, Anhui 230000, China.
J Pharm Biomed Anal. 2016 Sep 10;129:339-349. doi: 10.1016/j.jpba.2016.07.021. Epub 2016 Jul 15.
In China, ginger (Zingiberofficinale Rosc.) and its processed products, such as dried ginger and stir-frying ginger are commonly applied in traditional Chinese medicine (TCM). The paper presents the research on the effects of fresh ginger, dried ginger and stir-frying ginger extracts in blood stasis syndrome. First, a blood stasis syndrome rats model was established and then the hemorheological and blood coagulation activities were analyzed. Third, a sensitive, simple, and valid gas chromatography combined with time-of-flight mass spectrometry (GC-TOF/MS) method was established to compare the metabolic fingerprint coupled with multivariate analysis. The total 27 metabolites (16 in serum and 11 in urine) were identified and contributed to the blood stasis progress. These metabolites mainly involve six metabolism pathways in different impact-value. The altered efficacy index and metabolites can be regulated to normal levels by fresh ginger (FG), dried ginger (DG) and stir-frying ginger (SG). FG is the most effective as shown by the efficacy index, similarity analysis and peak intensity. The result presented here shows that metabolomics equipped with efficacy index makes it possible to study the blood stasis syndrome and to compare the effect and metabolites in fresh, dried and stir-frying gingers. The metabolomics approach can be recommended to study the pharmacological effect and mechanism of herbal drugs.
在中国，生姜（Zingiberofficinale Rosc.）及其加工产品，如干姜和炮姜，常用于传统中药（TCM）。本文介绍了鲜姜、干姜和炮姜提取物对血瘀证影响的研究。首先，建立血瘀证大鼠模型，然后分析血液流变学和凝血活性。第三，建立了一种灵敏、简单且有效的气相色谱-飞行时间质谱联用（GC-TOF/MS）方法，结合多变量分析比较代谢指纹图谱。共鉴定出27种代谢物（血清中16种，尿液中11种），这些代谢物促进了血瘀进展。这些代谢物主要涉及六个具有不同影响值的代谢途径。鲜姜（FG）、干姜（DG）和炮姜（SG）可将改变的疗效指标和代谢物调节至正常水平。从疗效指标、相似性分析和峰强度来看，鲜姜效果最佳。本文结果表明，结合疗效指标的代谢组学能够研究血瘀证，并比较鲜姜、干姜和炮姜的效果及代谢物。代谢组学方法可用于研究草药的药理作用和机制。
