Ningbo Research Institute, Zhejiang University, Ningbo 310027, China.
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Nutrients. 2020 Oct 19;12(10):3194. doi: 10.3390/nu12103194.
The beneficial effects of ginger polyphenols have been extensively reported. However, their metabolic characteristics and health effects on gut microbiota are poor understood. The purpose of this study was to investigate the digestion stability of ginger polyphenols and their prebiotic effects on gut microbiota by simulating digestion and fermentation in vitro. Following simulated digestion in vitro, 85% of the polyphenols were still detectable, and the main polyphenol constituents identified in ginger extract are 6-, 8-, and 10-gingerols and 6-shogaol in the digestive fluids. After batch fermentation, the changes in microbial populations were measured by 16S rRNA gene Illumina MiSeq sequencing. In mixed-culture fermentation with fecal inoculate, digested ginger extract (GE) significantly modulated the fecal microbiota structure and promoted the growth of some beneficial bacterial populations, such as and . Furthermore, incubation with GE could elevate the levels of short-chain fatty acids (SCFAs) accompanied by a decrease in the pH value. Additionally, the quantitative PCR results showed that 6-gingerol (6G), as the main polyphenol in GE, increased the abundance of significantly. Therefore, 6G is expected to be a potential prebiotic that improves human health by promoting gut health.
姜多酚的有益作用已被广泛报道。然而，它们的代谢特征和对肠道微生物群的健康影响还了解甚少。本研究旨在通过模拟体外消化和发酵来研究姜多酚的消化稳定性及其对肠道微生物群的益生元作用。经过体外模拟消化后，仍有 85%的多酚可检测到，姜提取物中的主要多酚成分是 6-、8-和 10-姜酚以及 6-姜烯酚。在分批发酵后，通过 16S rRNA 基因 Illumina MiSeq 测序来测量微生物种群的变化。在粪便接种物的混合培养发酵中，消化后的姜提取物（GE）显著调节了粪便微生物群的结构，并促进了一些有益细菌种群的生长，如 和 。此外，用 GE 孵育可以提高短链脂肪酸（SCFA）的水平，同时降低 pH 值。此外，定量 PCR 结果表明，6-姜酚（6G）作为 GE 中的主要多酚，显著增加了 的丰度。因此，6G 有望通过促进肠道健康成为一种改善人类健康的潜在益生元。
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