通过实时聚合酶链反应检测埃及流产山羊和绵羊中的流产布鲁氏菌DNA。
Detection of Brucella abortus DNA in aborted goats and sheep in Egypt by real-time PCR.
作者信息
Wareth Gamal, Melzer Falk, Tomaso Herbert, Roesler Uwe, Neubauer Heinrich
机构信息
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743, Jena, Germany.
Institute of Animal Hygiene and Environmental Health, Free University of Berlin, Robert-von-Ostertag Str. 7-13, 14163, Berlin, Germany.
出版信息
BMC Res Notes. 2015 Jun 3;8:212. doi: 10.1186/s13104-015-1173-1.
BACKGROUND
Brucellosis is a major zoonoses affects wide range of domesticated as well as wild animals. Despite the eradication program of brucellosis in Egypt, the disease is still endemic among cattle, buffaloes, sheep, goats, and camels.
RESULTS
In the present study, abortion occurred naturally among 25 animals (10 cows, 5 buffaloes, 9 Egyptian Baladi goats and 1 ewe) shared the same pasture were investigated by real-time polymerase chain reaction (RT-PCR). DNA of Brucella (B.) abortus was detected in serum of goats and sheep which has aborted recently by species-specific RT-PCR. The results suggest cross-species infection of B. abortus from cattle to non-preferred hosts raised in close contact.
CONCLUSION
This article will renew our knowledge about the Brucella agent causing abortion in small ruminants in Egypt. Information provided in this study is important for surveillance program, because eradication programs and vaccination strategies may have to be adapted accordingly.
背景
布鲁氏菌病是一种主要的人畜共患病，影响着广泛的家畜和野生动物。尽管埃及实施了布鲁氏菌病根除计划，但该病在牛、水牛、绵羊、山羊和骆驼中仍然流行。
结果
在本研究中，对在同一牧场自然流产的25只动物（10头奶牛、5头水牛、9只埃及巴拉迪山羊和1只母羊）进行了实时聚合酶链反应（RT-PCR）检测。通过种特异性RT-PCR在近期流产的山羊和绵羊血清中检测到布鲁氏菌流产亚种的DNA。结果表明，布鲁氏菌流产亚种存在从牛到密切接触饲养的非首选宿主的跨物种感染。
结论
本文将更新我们对埃及小型反刍动物流产布鲁氏菌病原体的认识。本研究提供的信息对监测计划很重要，因为根除计划和疫苗接种策略可能需要相应调整。
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