Fertrin Kleber Y, Samsel Leigh, van Beers Eduard J, Mendelsohn Laurel, Kato Gregory J, McCoy J Philip
Department of Clinical Pathology, School of Medical Sciences, University of Campinas, Campinas, Brazil.
Hematology and Hemotherapy Center-Hemocentro Campinas, University of Campinas, Campinas, Brazil.
Methods Mol Biol. 2016;1389:279-92. doi: 10.1007/978-1-4939-3302-0_20.
Hemoglobin S polymerization under hypoxic conditions in sickle cell disorders causes characteristic shape changes to human red blood cells. Previous sickling assays used to investigate the efficacy of novel agents to treat these disorders are laborious and observer dependent. Here, we describe a partially automated, high-throughput sickling assay using imaging flow cytometry.
镰状细胞疾病在缺氧条件下血红蛋白S聚合会导致人类红细胞出现特征性形状变化。以往用于研究新型药物治疗这些疾病疗效的镰变检测方法既费力又依赖观察者。在此，我们描述了一种使用成像流式细胞术的部分自动化高通量镰变检测方法。
