Moayedi Ali, Mora Leticia, Aristoy M-Concepción, Hashemi Maryam, Safari Mohammad, Toldrá Fidel
Department of Food Science and Technology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Instituto de Agroquimica y Technologia de Alimentos (CSIC), Avenue Agustin Escardino, Paterna, Valencia, Spain.
Appl Biochem Biotechnol. 2017 Jan;181(1):48-64. doi: 10.1007/s12010-016-2198-1. Epub 2016 Jul 26.
The effects of amino acid composition and peptide molecular mass on ACE-inhibitory and antioxidant activities of protein fragments obtained from tomato waste fermented using Bacillus subtilis were evaluated. The addition of B. subtilis increased the relative amounts of aromatic and positively-charged amino acids which have been described to influence the biological activities of peptide fragments. IC values of hydrolysates for ACE-inhibitory and 2, 2'-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities were found to be 1.5 and 8.2 mg/mL, respectively. Size-exclusion chromatography (SEC) pattern of the hydrolysate indicated the breakdown of parent proteins to smaller peptides with molecular weights mainly below 1400 Da. MALDI-TOF mass spectrometry analysis revealed that the highest ACE-inhibitory activity was due to peptides showing molecular mass range 500-800 Da, while the most active antioxidant peptides were found to be mainly at the two different peptide weight ranges 500-800 Da and 1200-1500 Da.
评估了氨基酸组成和肽分子量对利用枯草芽孢杆菌发酵番茄废料所得蛋白质片段的ACE抑制活性和抗氧化活性的影响。枯草芽孢杆菌的添加增加了芳香族和带正电荷氨基酸的相对含量，这些氨基酸已被描述为会影响肽片段的生物活性。水解产物对ACE抑制活性和2,2'-二苯基-1-苦基肼（DPPH）清除活性的IC值分别为1.5和8.2mg/mL。水解产物的尺寸排阻色谱（SEC）图谱表明，亲本蛋白质分解为分子量主要低于1400Da的较小肽段。基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）分析表明，最高的ACE抑制活性归因于分子量范围为500-800Da的肽段，而最具活性的抗氧化肽主要存在于500-800Da和1200-1500Da这两个不同的肽段分子量范围内。
