Institute of Bioresources and Sustainable Development, Regional Centre, Gangtok, India.
Centre of Innovative and Applied Bioprocessing, Mohali, India.
Bioresour Technol. 2022 May;351:126932. doi: 10.1016/j.biortech.2022.126932. Epub 2022 Mar 3.
A bioprocess was developed for production of bioactive peptides on microbial fermentation of rice beans using proteolytic Bacillus subtilis strains. The peptides produced were identified by LC-MS/MS analysis, revealing the presence of many unique peptide sequences to individual hydrolysates. On functional properties prediction, antihypertensive peptides (3.90%) were found to be higher in comparison to other bioactive peptides. Among different strains, B. subtilis KN2B fermented hydrolysate exhibited highest angiotensin converting enzyme (ACE)-inhibitory activity (45.73%). Furthermore, 19 selected peptides, including the common and unique peptides were examined for their affinity towards the binding cavity of ACE using molecular docking. The results showed a common peptide PFPIPFPIPIPLP, and another IPFPPIPFLPPI unique to B. subtilis KN2B fermented hydrolysate exhibited promising binding at the ACE binding site with substantial free binding energy. The process developed can be used for the production of bioactive peptides from rice bean for application in nutraceutical industries.
采用蛋白酶枯草芽孢杆菌菌株对饭豆进行微生物发酵，开发了一种生产生物活性肽的生物工艺。通过 LC-MS/MS 分析鉴定所产生的肽，揭示了各个水解物中存在许多独特的肽序列。在功能特性预测中，发现降压肽（3.90%）比其他生物活性肽更高。在不同的菌株中，枯草芽孢杆菌 KN2B 发酵的水解物表现出最高的血管紧张素转化酶（ACE）抑制活性（45.73%）。此外，还使用分子对接研究了 19 种选定的肽，包括常见和独特的肽，以研究它们与 ACE 结合腔的亲和力。结果表明，一种常见的肽 PFPIPFPIPIPLP 和另一种独特的 IPFPPIPFLPPI 来自枯草芽孢杆菌 KN2B 发酵的水解物，在 ACE 结合部位表现出有希望的结合，具有可观的自由结合能。所开发的工艺可用于从饭豆生产生物活性肽，应用于营养保健品行业。
