Jacob Tharun Varghese, Ramesh Maya, Murali S, Ramesh K, Sanjay P R, Abraham Philip
Department of Oral and Maxillofacial Pathology, Sri Sankara Dental College, Trivandrum, Kerala, India.
Department of Oral Pathology, Vinayaka Missions, Sankarachariyar Dental College, Salem, Tamil Nadu, India.
J Cancer Res Ther. 2016 Apr-Jun;12(2):634-9. doi: 10.4103/0973-1482.148697.
Sialic acids are important terminal sugars of the carbohydrate chains of cell membrane glycoproteins and glycolipids. The progressive rise in total sialic acid (TSA) levels has been significantly associated with tumor progression and metastasis.
Samples of 2 ml of unstimulated whole saliva were collected from 20 healthy patients, and 20 cases of histopathologically confirmed oral pre-cancer patients and 20 cases of histopathologically confirmed oral squamous cell carcinoma (OSCC) patients, using the method of Navazesh. Estimation of total sialic acid was done by the histochemical method of Yao et al.
The study revealed significantly elevated total sialic acid (TSA) levels in the saliva of oral precancer and OSCC patients, yielding a mean value of 59.75 ± 7.29 mg/dl and 204.85 ± 60.38 mg/dl, respectively, against the mean value of 21.65 ± 5.71 mg/dl of the healthy controls. Also, high total sialic acid (TSA) levels in the well-differentiated squamous cell carcinoma cases were appreciable to those of moderately differentiated ones.
The result suggests the correlation of elevated salivary TSA levels with the progression of OSCC. In conclusion, the study indicates that the glycoprotein metabolism is significantly altered in the saliva of patients, with both oral pre-cancer and OSCC.
唾液酸是细胞膜糖蛋白和糖脂碳水化合物链的重要末端糖。总唾液酸（TSA）水平的逐渐升高与肿瘤进展和转移显著相关。
采用纳瓦泽什方法，从20名健康患者、20例经组织病理学确诊的口腔癌前病变患者和20例经组织病理学确诊的口腔鳞状细胞癌（OSCC）患者中采集2ml未刺激的全唾液样本。总唾液酸的测定采用姚等人的组织化学方法。
研究显示，口腔癌前病变患者和OSCC患者唾液中的总唾液酸（TSA）水平显著升高，平均值分别为59.75±7.29mg/dl和204.85±60.38mg/dl，而健康对照组的平均值为21.65±5.71mg/dl。此外，高分化鳞状细胞癌病例中的总唾液酸（TSA）水平与中分化病例相当。
结果表明唾液TSA水平升高与OSCC进展相关。总之，该研究表明，口腔癌前病变患者和OSCC患者唾液中的糖蛋白代谢发生了显著改变。
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