Colleges of Marine Life Sciences, Ocean University of China, Qingda 266003, China.
Fish Shellfish Immunol. 2012 Mar;32(3):391-5. doi: 10.1016/j.fsi.2011.11.014. Epub 2011 Dec 8.
In this study, we investigated the immune enhancing effects of different adjuvants used in a pentavalent vaccine for turbots. The pentavalent vaccine consisted of inactive bacterial cells from five common pathogenic strains (Vibrio anguillarum, Vibrio scophtalmi, Edwardsiella tarda, Vibrio harveyi and Vibrio alginolyticus) and the adjuvants were astragalus polysaccharides (APS), propolis, and the Freund's complete adjuvant (FCA). Turbots were immunized with the pentavalent vaccine alone or with one of the adjuvants, and the immune efficiency was evaluated by measuring the activities of lysozyme (LSZ) and superoxide dismutase (SOD), and serum antibody titers. Fish were also challenged with the pathogens after immunization and the relative percent survival (RPS) was assessed. Our results showed that APS, propolis, and FCA had significant immune-enhancing effects on turbots as shown by the higher titers of antibodies against the pathogens, increased LSZ and SOD activities, and enhanced RPS after challenge with pathogens. Among the three adjuvants, FCA had the most significant immune synergistic effects with the vaccine, and APS and propolis had lower and similar immune synergies.
在这项研究中，我们研究了不同佐剂在鲆鱼五价疫苗中的免疫增强作用。五价疫苗由五种常见致病菌（鳗弧菌、灿烂弧菌、迟缓爱德华氏菌、哈维氏弧菌和溶藻弧菌）的非活性细菌细胞和佐剂黄芪多糖（APS）、蜂胶和弗氏完全佐剂（FCA）组成。我们用五价疫苗单独或用一种佐剂对鲆鱼进行免疫，并通过测量溶菌酶（LSZ）和超氧化物歧化酶（SOD）的活性以及血清抗体滴度来评估免疫效率。免疫后，我们还用病原体对鱼进行攻毒，并评估相对存活百分率（RPS）。我们的结果表明，APS、蜂胶和 FCA 对鲆鱼具有显著的免疫增强作用，表现为对病原体的抗体滴度更高、LSZ 和 SOD 活性增加，以及对病原体攻毒后的 RPS 增强。在这三种佐剂中，FCA 与疫苗具有最显著的免疫协同作用，而 APS 和蜂胶的免疫协同作用较低且相似。
