Niemirowicz Katarzyna, Durnaś Bonita, Tokajuk Grażyna, Głuszek Katarzyna, Wilczewska Agnieszka Z, Misztalewska Iwona, Mystkowska Joanna, Michalak Grzegorz, Sodo Anna, Wątek Marzena, Kiziewicz Bożena, Góźdź Stanisław, Głuszek Stanisław, Bucki Robert
Department of Microbiological and Nanobiomedical Engineering, Medical University of Białystok, Białystok, Poland.
Holy Cross Oncology Center of Kielce, Kielce, Poland.
Nanomedicine. 2016 Nov;12(8):2395-2404. doi: 10.1016/j.nano.2016.07.006. Epub 2016 Jul 25.
This study was designed to assess the antifungal/anti-biofilm and hemolytic properties of two polyene antibiotics, amphotericin B (AMF) and nystatin (NYS), attached to the surface of magnetic nanoparticles (MNP) against clinical isolates of Candida species and human red blood cells, respectively. The developed nanosystems, MNP@AMF and MNP@NYS, displayed stronger fungicidal activity than unbound AMF or NYS. Synergistic activity was observed with a combination of polyenes and MNPs against all tested Candida strains. Nanosystems were more potent than unbound agents when tested against Candida strains in the presence of pus, and as agents able to prevent Candida biofilm formation. The observed inactivation of catalase Cat1 in Candida cells upon treatment with the nanosystems suggests that disruption of the oxidation-reduction balance is a mechanism leading to inhibition of Candida growth. The significant decrease of polyenes lytic activity against host cells after their attachment to MNPs surface indicates improvement in their biocompatibility.
本研究旨在评估附着于磁性纳米颗粒（MNP）表面的两种多烯抗生素两性霉素B（AMF）和制霉菌素（NYS）分别针对念珠菌临床分离株和人类红细胞的抗真菌/抗生物膜及溶血特性。所研发的纳米系统MNP@AMF和MNP@NYS表现出比未结合的AMF或NYS更强的杀菌活性。多烯类药物与MNPs联合使用时，对所有测试的念珠菌菌株均观察到协同活性。当在脓液存在的情况下针对念珠菌菌株进行测试时，纳米系统比未结合的药物更有效，并且作为能够防止念珠菌生物膜形成的药物。在用纳米系统处理后，观察到念珠菌细胞中的过氧化氢酶Cat1失活，这表明氧化还原平衡的破坏是导致念珠菌生长受到抑制的一种机制。多烯类药物附着于MNPs表面后对宿主细胞的裂解活性显著降低，表明其生物相容性得到改善。
