Tabbene Olfa, Di Grazia Antonio, Azaiez Sana, Ben Slimene Imen, Elkahoui Salem, Alfeddy Mohamed Najib, Casciaro Bruno, Luca Vincenzo, Limam Ferid, Mangoni Maria Luisa
Laboratory of Bioactive Substances, Center of Biotechnology, Ecopark of Borj Cedria, Hammam-Lif, Tunisia
Pasteur Institute-Cenci Bolognetti Foundation, Department of Biochemical Sciences, Sapienza University of Rome, Italy.
FEMS Yeast Res. 2015 Jun;15(4):fov022. doi: 10.1093/femsyr/fov022. Epub 2015 May 7.
In the present study, the synergism of the lipopeptide bacillomycin D in combination with the polyene amphotericin B against pathogenic Candida species is described along with their potential cytotoxicity against mammalian cells. Bacillomycin D inhibited the growth of various Candida species at minimal concentrations from 12.5 to 25 μg ml(-1). Furthermore, it showed a synergistic effect with the antifungal drug amphotericin B in inhibiting the growth of Candida strains, with fractional inhibitory concentration indices ranging from 0.28 to 0.5. Time killing studies revealed a >2-log reduction in the viability of Candida albicans ATCC 10231 cells after 3 h incubation with the combination amphotericin B plus bacillomycin D, at their subinhibitory concentration. Interestingly, when the two drugs were used together at those dosages displaying a synergism in the anti-Candida activity, no cytotoxic effect was observed against mammalian cells. Therefore, the combination bacillomycin D/amphotericin B may represent a valid alternative to conventional antifungals for topical treatment of C. albicans infections. To the best of our knowledge, this is the first report describing the in vitro interaction between the antifungal drug amphotericin B and bacillomycin D against pathogenic Candida species.
在本研究中，描述了脂肽杆菌霉素D与多烯两性霉素B联合使用对致病性念珠菌的协同作用及其对哺乳动物细胞的潜在细胞毒性。杆菌霉素D在12.5至25μg/ml的最低浓度下抑制了各种念珠菌的生长。此外，它与抗真菌药物两性霉素B在抑制念珠菌菌株生长方面显示出协同作用，部分抑制浓度指数范围为0.28至0.5。时间杀菌研究表明，在亚抑菌浓度下，白色念珠菌ATCC 10231细胞与两性霉素B加杆菌霉素D联合孵育3小时后，其活力降低了>2个对数。有趣的是，当这两种药物以在抗念珠菌活性中显示协同作用的剂量一起使用时，未观察到对哺乳动物细胞的细胞毒性作用。因此，杆菌霉素D/两性霉素B组合可能是局部治疗白色念珠菌感染的传统抗真菌药物的有效替代品。据我们所知，这是第一份描述抗真菌药物两性霉素B和杆菌霉素D对致病性念珠菌体外相互作用的报告。
