斑马鱼中的化学筛选

Chemical Screening in Zebrafish.

作者信息

Brady Colleen A, Rennekamp Andrew J, Peterson Randall T

机构信息

Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, 149 13th Street, Charlestown, MA, 02129, USA.

Department of Systems Biology, Harvard Medical School, 200 Longwood Ave, Boston, MA, 02115, USA.

出版信息

Methods Mol Biol. 2016;1451:3-16. doi: 10.1007/978-1-4939-3771-4_1.

DOI:10.1007/978-1-4939-3771-4_1

PMID:27464797

Abstract

Phenotypic small molecule screens in zebrafish have gained popularity as an unbiased approach to probe biological processes. In this chapter we outline basic methods for performing chemical screens with larval zebrafish including breeding large numbers of embryos, plating larval fish into multi-well dishes, and adding small molecules to these wells. We also highlight important considerations when designing and interpreting the results of a phenotypic screen and possible follow-up approaches, including popular methods used to identify the mechanism of action of a chemical compound.

摘要

斑马鱼中的表型小分子筛选作为一种探究生物过程的无偏倚方法已受到广泛关注。在本章中，我们概述了对斑马鱼幼体进行化学筛选的基本方法，包括大量胚胎的繁殖、将幼体鱼接种到多孔培养皿中以及向这些孔中添加小分子。我们还强调了在设计和解释表型筛选结果时的重要注意事项以及可能的后续方法，包括用于确定化合物作用机制的常用方法。