Chen S Y
Pathology Department, Temple University School of Medicine, Philadelphia, PA 19140.
J Oral Pathol Med. 1989 Feb;18(2):108-12. doi: 10.1111/j.1600-0714.1989.tb00745.x.
Fifteen male and 15 female HMT rats, 6 months of age, received weekly applications of smokeless tobacco to the buccal mucosa for one year. Hyperorthokeratosis, acanthosis, numerous binucleate spinous cells, and subepithelial connective tissue hyalinization were observed, whereas verrucous carcinoma and squamous cell carcinoma were not seen. Karyotyping revealed that lymphocytes of tobacco-treated as well as control rats had normal chromosome number (2N = 42) and morphology. However, approximately 25% of buccal epithelial cells of the tobacco-treated rats were tetraploid and 5% octaploid, compared to only 11% tetraploid and no octaploid in the controls. Results indicate that the effects of smokeless tobacco on the rat buccal mucosa are similar to those observed in humans and that the mitotic process can be disturbed by tobacco treatment.
15只6月龄雄性和15只6月龄雌性HMT大鼠，每周一次在颊黏膜涂抹无烟烟草，持续一年。观察到过度正角化、棘层肥厚、大量双核棘细胞以及上皮下结缔组织玻璃样变，但未发现疣状癌和鳞状细胞癌。核型分析显示，经烟草处理的大鼠以及对照大鼠的淋巴细胞染色体数目（2N = 42）和形态均正常。然而，经烟草处理的大鼠约25%的颊上皮细胞为四倍体，5%为八倍体，而对照组仅11%为四倍体，无八倍体。结果表明，无烟烟草对大鼠颊黏膜的影响与在人类中观察到的相似，并且烟草处理可干扰有丝分裂过程。
