Prasad Kavitha, Rao Roopa, Augustine Dominic, Sowmya Samudrala Venkatesiah, Haragannavar Vanishri, Sagar Parimala, Sreedhar Prathibha
MDS, Department of Oral and Maxillofacial Surgery, Faculty of Dental Sciences, Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India.
MDS, Department of Oral and Maxillofacial Pathology, Faculty of Dental Sciences, Ramaiah University of Applied Sciences, Bengaluru, Karnataka, India.
Head Neck. 2019 Feb;41(2):388-397. doi: 10.1002/hed.25494. Epub 2018 Dec 11.
The objective was to study comprehensive mRNA expression profiles of buccal mucosa oral squamous cell carcinoma (OSCC-BM) and gingivo-buccal OSCC (OSCC-GB) in smokeless tobacco chewers to understand the biological behavior of OSCC at these specific sites and identify diagnostic and prognostic markers.
High throughput RNA sequencing transcriptome of fresh buccal mucosa (4 samples) and gingivo-buccal (4 samples) OSCC with normal oral mucosa (3 samples) was performed on Illumina NextSeq500 paired end sequencing with 75x2bp.
In the comparison between OSCC and normal, there were 402 differentially expressed genes (DEGs); between OSCC-BM and normal, there were 467 DEGs; and between OSCC-GB and normal oral tissue, there were 608 DEGs. Pathway-based analysis of gene expression was done. The inflammation mediated by chemokine and cytokine signaling pathway had the maximum gene hits.
FZD2 and its interactions with the cadherins have a role in invasion and metastasis. immunosurveillance is evident in OSCC-GB with the downregulation of CADM1.
目的是研究无烟烟草咀嚼者颊黏膜口腔鳞状细胞癌（OSCC - BM）和牙龈颊侧OSCC（OSCC - GB）的综合mRNA表达谱，以了解OSCC在这些特定部位的生物学行为，并确定诊断和预后标志物。
对新鲜颊黏膜（4个样本）、牙龈颊侧（4个样本）OSCC以及正常口腔黏膜（3个样本）进行高通量RNA测序转录组分析，采用Illumina NextSeq500进行75x2bp双端测序。
在OSCC与正常组织的比较中，有402个差异表达基因（DEG）；在OSCC - BM与正常组织的比较中，有467个DEG；在OSCC - GB与正常口腔组织的比较中，有608个DEG。进行了基于通路的基因表达分析。趋化因子和细胞因子信号通路介导的炎症反应有最多的基因命中数。
FZD2及其与钙黏蛋白的相互作用在侵袭和转移中起作用。CADM1的下调表明OSCC - GB存在免疫监视。
