Zhang M, Zhou Y-F, Gong J-Y, Gao C-B, Li S-L
Department of Ophthalmology, The First Affiliated Hospital of AnHui Medical University, AnHui Hefei, China.
Eur Rev Med Pharmacol Sci. 2016 Jul;20(15):3152-60.
Dysfunction of autophagy has been implicated in development and progression of diverse human cancers. However, the exact role and mechanism of autophagy have not been fully understood in human cancers, especially in retinoblastoma (Rb).
We determined the autophagy activity in human Rb tissues by assessing the autophagy markers microtubule-associated protein light chain 3B (LC3) and p62 (SQSTM1) in formalin fixed and paraffin embedded human tissue by immunohistochemistry and then associated their expression with patient clinicopathological features. We further explored the correlation between the expression of LC3B and p62 and the expression of cytoplasmic p53, a newly identified autophagy suppressor, in Rb tissues.
Our data revealed that the expression of LC3B and p62, was significantly associated with disease progression and tumor invasion of Rb. Furthermore, we also revealed that cytoplasmic expression of p53 was inversely associated with the behavior of tumor invasion. Finally, Spearman correlation analysis demonstrated that cytoplasmic expression of p53 was significantly and inversely correlated to the expression of both LC3B and p62.
Autophagy might play an important role in human Rb progression, and LC3B and p62 may be useful predictors of disease progression in patients with Rb.
自噬功能障碍与多种人类癌症的发生和发展有关。然而，自噬在人类癌症，尤其是视网膜母细胞瘤（Rb）中的具体作用和机制尚未完全明确。
我们通过免疫组织化学评估福尔马林固定石蜡包埋的人体组织中的自噬标志物微管相关蛋白轻链3B（LC3）和p62（SQSTM1），从而确定人Rb组织中的自噬活性，然后将它们的表达与患者的临床病理特征相关联。我们进一步探讨了Rb组织中LC3B和p62的表达与新发现的自噬抑制因子细胞质p53表达之间的相关性。
我们的数据显示，LC3B和p62的表达与Rb的疾病进展和肿瘤侵袭显著相关。此外，我们还发现p53的细胞质表达与肿瘤侵袭行为呈负相关。最后，Spearman相关性分析表明，p53的细胞质表达与LC3B和p62的表达均呈显著负相关。
自噬可能在人Rb进展中起重要作用，LC3B和p62可能是Rb患者疾病进展的有用预测指标。
