de Souza E G, Hara C C P, Fagundes D L G, de Queiroz A A, Morceli G, Calderon I M P, França E L, Honorio-França A C
Institute of Biological and Health Science, Federal University of Mato Grosso, Barra do Garças, MT, Brazil.
Graduate Program in Gynecology, Obstetrics and Mastology, Botucatu Medical School, São Paulo State University/UNESP, Botucatu, SP, Brazil.
Scand J Immunol. 2016 Oct;84(4):237-44. doi: 10.1111/sji.12466.
This study investigated the expression of the neonatal Fc receptor (FcRn) in maternal blood, cord blood and placental cells and determined IgG levels in maternal blood and cord blood from diabetic mothers. Peripheral blood, cord blood and placenta samples were collected from 26 mothers with normoglycaemia (non-diabetic, ND group) and 52 with hyperglycaemia (26 with mild gestational hyperglycaemia, MGH group, and 26 with type 2 diabetes mellitus, DM-2 group). Cells expressing CD19(+) and FcRn were identified by flow cytometry. Total IgG and its subclasses were quantified by ELISA. Maternal blood from DM-2 and cord blood from MGH exhibited a higher proportion of CD19(+) expression by B cells. DM-2 showed a lower proportion of CD19(+) cells in placenta. FcRn expression increased in cells from cord blood and placenta from MGH. Maternal blood, cord blood and placenta cells from DM-2 showed lower FcRn expression. Blood IgG levels were lower in DM-2, and cord blood IgG levels were higher in MGH. The highest levels of IgG4 were detected in the blood of hyperglycaemic mothers. The highest IgG3 and IgG4 levels in cord blood were detected in MGH, and the lowest IgG2 and IgG3 levels in DM-2. Maternal hyperglycaemia compromised placental transfer of IgG1, IgG3 and IgG4. The results suggest that regardless of hyperglycaemia degree, it decreases FcRn expression in placenta and blood cells and compromises the production and transfer of antibodies from maternal blood to newborns.
本研究调查了新生儿Fc受体（FcRn）在母体血液、脐带血和胎盘细胞中的表达，并测定了糖尿病母亲的母体血液和脐带血中的IgG水平。从26名血糖正常的母亲（非糖尿病，ND组）和52名血糖升高的母亲（26名轻度妊娠高血糖，MGH组，26名2型糖尿病，DM - 2组）中采集外周血、脐带血和胎盘样本。通过流式细胞术鉴定表达CD19(+)和FcRn的细胞。通过ELISA定量总IgG及其亚类。DM - 2组的母体血液和MGH组的脐带血中B细胞表达CD19(+)的比例较高。DM - 2组胎盘组织中CD19(+)细胞比例较低。MGH组脐带血和胎盘细胞中FcRn表达增加。DM - 2组的母体血液、脐带血和胎盘细胞中FcRn表达较低。DM - 2组血液IgG水平较低，MGH组脐带血IgG水平较高。在高血糖母亲的血液中检测到最高水平的IgG4。MGH组脐带血中IgG3和IgG4水平最高，DM - 2组中IgG2和IgG3水平最低。母体高血糖会损害IgG1、IgG3和IgG4的胎盘转运。结果表明，无论高血糖程度如何，它都会降低胎盘和血细胞中FcRn的表达，并损害抗体从母体血液向新生儿的产生和转运。
