Gao Ziqing, Yuan Yue, Xu Lei, Liu Ran, Chen Ming, Zhang Chunzhi
School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, PR China.
Int J Syst Evol Microbiol. 2016 Oct;66(10):4185-4190. doi: 10.1099/ijsem.0.001333. Epub 2016 Jul 27.
A novel bacterium, designated strain CF1T, was isolated from a soil sample of a tea plantation and its taxonomic position was determined using a polyphasic approach. Strain CF1T was a Gram-stain-negative, facultatively anaerobic, non-sporulating, non-motile and rod-shaped bacterium. Optimum growth occurred at 25 °C and pH 6.0. Comparative analysis of the 16S rRNA gene sequence showed that the isolate belongs to the genus Paraburkholderia, showing highest levels of similarity with respect to Paraburkholderia sediminicola LMG 24238T (98.44 %). Additionally, strain CF1T, P. sediminicola LMG 24238T and Paraburkholderia aspalathi LMG 27731 formed a distinct group in the phylogenetic tree based on 16S rRNA gene sequences. The predominant ubiquinone was Q-8, and the polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified aminophospholipid, two unidentified aminolipids and two unidentified polar lipids. The DNA G+C content was 60.2 mol%, and the major fatty acids were C16 : 0, summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The DNA-DNA relatedness values between strain CF1T and its close relatives including P. sediminicola LMG 24238T and P. aspalathi LMG 27731 49.3±0.4 % and 38.3±0.5 %, respectively. On the basis of phylogenetic analysis, phenotypic and genotypic data, it is concluded that the isolate represents a novel species of the genus Paraburkholderia, for which the name Paraburkholderia caffeinilytica sp. nov. is proposed. The type strain is CF1T (=LMG 28690T=CGMCC 1.15103T).
从茶园土壤样本中分离出一株新细菌，命名为CF1T菌株，并采用多相分类法确定其分类地位。CF1T菌株是革兰氏染色阴性、兼性厌氧、不产芽孢、无运动性的杆状细菌。最适生长温度为25℃，最适pH值为6.0。16S rRNA基因序列的比较分析表明，该分离株属于类伯克霍尔德氏菌属，与栖泥类伯克霍尔德氏菌LMG 24238T相似度最高（98.44%）。此外，基于16S rRNA基因序列，CF1T菌株、栖泥类伯克霍尔德氏菌LMG 24238T和阿斯帕拉类伯克霍尔德氏菌LMG 27731在系统发育树中形成一个独特的分支。主要泛醌为Q-8，极性脂质谱由磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、一种未鉴定的氨基磷脂、两种未鉴定的氨基脂质和两种未鉴定的极性脂质组成。DNA的G+C含量为60.2 mol%，主要脂肪酸为C16 : 0、总和特征8（C18 : 1ω7c和/或C18 : 1ω6c）和总和特征3（C16 : 1ω7c和/或C16 : 1ω6c）。CF1T菌株与其近缘菌株（包括栖泥类伯克霍尔德氏菌LMG 24238T和阿斯帕拉类伯克霍尔德氏菌LMG 27731）之间的DNA-DNA相关性值分别为49.3±0.4%和38.3±0.5%。基于系统发育分析、表型和基因型数据，得出该分离株代表类伯克霍尔德氏菌属的一个新物种的结论，并为此提出新名称咖啡类伯克霍尔德氏菌（Paraburkholderia caffeinilytica sp. nov.）。模式菌株为CF1T（=LMG 28690T=CGMCC 1.15103T）。
