Gao Ziqing, Su Chenglin, Yang Xueying, Sun Di, Zeng Chao, Chen Ming, Hu Wenzhong, Zhang Chunzhi
School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, PR China.
College of Life Science, Dalian Minzu University, Dalian 116600, PR China.
Int J Syst Evol Microbiol. 2017 Dec;67(12):4962-4966. doi: 10.1099/ijsem.0.002358. Epub 2017 Oct 16.
A novel bacterium, designated strain DL503, was isolated from a Daqu sample and its taxonomic position determined using a polyphasic taxonomy. Strain DL503 was a Gram-stain-negative, facultatively anaerobic, non-sporulating, motile and coccoid-rod-shaped bacterium. Optimum growth occurred at 20-45 °C, pH 5.0-10.0 and 1.5 % (w/v) NaCl. Comparative analysis of the 16S rRNA gene sequence showed that the isolate belongs to the genus Franconibacter, showing highest levels of similarity with respect to Franconibacter pulveris JCM 16471 (98.94 %) and Franconibacter helveticus DSM 18396 (98.39 %). Cells contained the quinones Q-8 and MK-8, and the polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, three unidentified polar lipids and three unidentified amino lipids. The DNA G+C content was 53.3 mol% and the major fatty acids were C16 : 0, C17 : 0 cyclo, summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), summed feature 4 (C17 : 1 iso I and/or C17 : 1 anteiso B) and summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c). The DNA-DNA relatedness values between strain DL503 and its close relatives, including F. pulveris JCM 16471 and F. helveticus DSM 18396, were 51.5±0.5 % and 45.2±1.1 %, respectively. Based on phylogenetic analysis, phenotypic and genotypic data, it is concluded that the isolate represents a novel species of the genus Franconibacter, for which the name Franconibacter daqui sp. nov. is proposed. The type strain is DL503 (=LMG 29914=CGMCC 1.15944).
从大曲样品中分离出一株新型细菌，命名为菌株DL503，并采用多相分类法确定其分类地位。菌株DL503是革兰氏阴性、兼性厌氧、无芽孢、具运动性的球状杆菌。在20-45 °C、pH 5.0-10.0和1.5 %（w/v）NaCl条件下生长最佳。16S rRNA基因序列的比较分析表明，该分离株属于弗朗科尼氏菌属，与粉状弗朗科尼氏菌JCM 16471（98.94 %）和瑞士弗朗科尼氏菌DSM 18396（98.39 %）的相似性最高。细胞含有醌Q-8和MK-8，极性脂质谱由磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、三种未鉴定的极性脂质和三种未鉴定的氨基脂质组成。DNA G+C含量为53.3 mol%，主要脂肪酸为C16 : 0、C17 : 0环丙烷、总和特征3（C16 : 1 ω7c和/或C16 : 1 ω6c）、总和特征4（C17 : 1异戊烯基I和/或C17 : 1前异戊烯基B）和总和特征8（C18 : 1 ω7c和/或C18 : 1 ω6c）。菌株DL503与其近缘菌株，包括粉状弗朗科尼氏菌JCM 16471和瑞士弗朗科尼氏菌DSM 18396之间的DNA-DNA相关性值分别为51.5±0.5 %和45.2±1.1 %。基于系统发育分析、表型和基因型数据，得出该分离株代表弗朗科尼氏菌属的一个新物种的结论，为此提出新名称粉状弗朗科尼氏菌（Franconibacter daqui sp. nov.）。模式菌株为DL503（=LMG 29914=CGMCC 1.15944）。
