中国仓鼠卵巢细胞突变体中胆固醇生物合成和质膜流动性的调节缺陷。
Defective regulation of cholesterol biosynthesis and plasma membrane fluidity in a Chinese hamster ovary cell mutant.
作者信息
Sinensky M
出版信息
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1247-9. doi: 10.1073/pnas.75.3.1247.
Abstract
A Chinese hamster ovary cell mutant resistant to killing by 25-hydroxycholesterol is shown to be defective in the regulation of cholesterol synthesis by exogenous cholesterol. When grown with various cholesterol supplements in delipidized serum, the mutant cell exhibits a variation in cholesterol content and plasma membrane fluidity that is not observed in the parental cell type.
摘要
一种对25-羟基胆固醇杀伤具有抗性的中国仓鼠卵巢细胞突变体，被证明在通过外源性胆固醇调节胆固醇合成方面存在缺陷。当在脱脂血清中添加各种胆固醇补充剂培养时，该突变体细胞的胆固醇含量和质膜流动性会发生变化，而在亲代细胞类型中未观察到这种变化。
相似文献
1
Defective regulation of cholesterol biosynthesis and plasma membrane fluidity in a Chinese hamster ovary cell mutant.中国仓鼠卵巢细胞突变体中胆固醇生物合成和质膜流动性的调节缺陷。
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1247-9. doi: 10.1073/pnas.75.3.1247.
2
Adaptive alteration in phospholipid composition of plasma membranes from a somatic cell mutant defective in the regulation of cholesterol biosynthesis.来自胆固醇生物合成调节缺陷的体细胞突变体的质膜磷脂组成的适应性改变。
J Cell Biol. 1980 Apr;85(1):166-9. doi: 10.1083/jcb.85.1.166.
3
Altered interaction between Sendai virus and a Chinese hamster cell mutant with defective cholesterol synthesis.
Biochim Biophys Acta. 1987 Nov 2;904(1):159-64. doi: 10.1016/0005-2736(87)90099-x.
4
Plasma membrane cholesterol: a possible barrier to intracellular oxygen in normal and mutant CHO cells defective in cholesterol metabolism.质膜胆固醇：在胆固醇代谢缺陷的正常和突变CHO细胞中，可能是细胞内氧气的屏障。
Biochemistry. 2003 Jan 14;42(1):23-9. doi: 10.1021/bi026039t.
5
86Rb+ fluxes in Chinese hamster ovary cells as a function of membrane cholesterol content.
Biochim Biophys Acta. 1979 Dec 12;558(3):296-306. doi: 10.1016/0005-2736(79)90264-5.
6
Rate limitation of (Na+ + K+)-stimulated adenosinetriphosphatase by membrane acyl chain ordering.
Proc Natl Acad Sci U S A. 1979 Oct;76(10):4893-7. doi: 10.1073/pnas.76.10.4893.
7
Recurrent G-to-A substitution in a single codon of SREBP cleavage-activating protein causes sterol resistance in three mutant Chinese hamster ovary cell lines.固醇调节元件结合蛋白裂解激活蛋白单密码子中的反复G到A替换在三种突变的中国仓鼠卵巢细胞系中导致固醇抗性。
Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13709-14. doi: 10.1073/pnas.93.24.13709.
8
The regulation of membrane lipid fluidity by membrane lipid biosynthesis.膜脂生物合成对膜脂流动性的调节。
Adv Pathobiol. 1980;7:365-76.
9
The fluidity of Chinese hamster ovary cell and bull sperm membranes after cholesterol addition.添加胆固醇后中国仓鼠卵巢细胞和公牛精子膜的流动性
Cryobiology. 2005 Aug;51(1):102-12. doi: 10.1016/j.cryobiol.2005.05.004.
10
[Cultured cell mutants defective in synthesis of membrane lipids: a key tool for studies on membrane lipid domains].[膜脂合成缺陷的培养细胞突变体：膜脂结构域研究的关键工具]
Tanpakushitsu Kakusan Koso. 2002 Mar;47(4 Suppl):425-30.
引用本文的文献
1
Proton NMR visible mobile lipid signals in sensitive and multidrug-resistant K562 cells are modulated by rafts.质子核磁共振可见的敏感及多药耐药K562细胞中的流动脂质信号受脂筏调节。
Cancer Cell Int. 2005 Feb 9;5(1):2. doi: 10.1186/1475-2867-5-2.
2
Adaptive alteration in phospholipid composition of plasma membranes from a somatic cell mutant defective in the regulation of cholesterol biosynthesis.来自胆固醇生物合成调节缺陷的体细胞突变体的质膜磷脂组成的适应性改变。
J Cell Biol. 1980 Apr;85(1):166-9. doi: 10.1083/jcb.85.1.166.
3
Lipid vesicle-mediated alterations of membrane cholesterol levels: effects on Na+ and K+ currents in squid axon.脂质囊泡介导的膜胆固醇水平改变：对鱿鱼轴突中Na+和K+电流的影响。
J Membr Biol. 1981;63(3):191-8. doi: 10.1007/BF01870980.
4
Effect of sterol side chains on growth and membrane fatty acid composition of Saccharomyces cerevisiae.甾醇侧链对酿酒酵母生长及膜脂肪酸组成的影响
J Bacteriol. 1980 Oct;144(1):124-30. doi: 10.1128/jb.144.1.124-130.1980.
5
Surface morphological study of Ehrlich ascites tumor cells exposed to microwave irradiation and heat.暴露于微波辐射和热的艾氏腹水癌细胞的表面形态学研究。
Experientia. 1984 Aug 15;40(8):881-4. doi: 10.1007/BF01952008.
6
Activation of the silent endogenous cholesterol-7-alpha-hydroxylase gene in rat hepatoma cells: a new complementation group having resistance to 25-hydroxycholesterol.大鼠肝癌细胞中沉默的内源性胆固醇7-α-羟化酶基因的激活：一个对25-羟胆固醇具有抗性的新互补群。
Mol Cell Biol. 1991 Apr;11(4):2049-56. doi: 10.1128/mcb.11.4.2049-2056.1991.
7
Rate limitation of (Na+ + K+)-stimulated adenosinetriphosphatase by membrane acyl chain ordering.
Proc Natl Acad Sci U S A. 1979 Oct;76(10):4893-7. doi: 10.1073/pnas.76.10.4893.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM.哺乳动物细胞在化学成分明确的合成培养基中的克隆生长
Proc Natl Acad Sci U S A. 1965 Feb;53(2):288-93. doi: 10.1073/pnas.53.2.288.
3
A rapid method of total lipid extraction and purification.一种快速的总脂质提取与纯化方法。
Can J Biochem Physiol. 1959 Aug;37(8):911-7. doi: 10.1139/o59-099.
4
Phosphorus assay in column chromatography.柱色谱法中的磷测定
J Biol Chem. 1959 Mar;234(3):466-8.
5
Cholesterol requirement of primary diploid human fibroblasts.原代二倍体人成纤维细胞的胆固醇需求
J Cell Biol. 1969 Jul;42(1):262-71. doi: 10.1083/jcb.42.1.262.
6
Molecular motion in spin-labeled phospholipids and membranes.自旋标记磷脂和膜中的分子运动。
J Am Chem Soc. 1971 Jan 27;93(2):314-26. doi: 10.1021/ja00731a005.
7
Genetics of somatic mammalian cells. IX. Quantitation of mutagenesis by physical and chemical agents.哺乳动物体细胞遗传学。IX. 物理和化学因子诱变的定量分析。
J Cell Physiol. 1969 Dec;74(3):245-58. doi: 10.1002/jcp.1040740305.
8
Regulation of cholesterol metabolism. I.胆固醇代谢的调节。I.
N Engl J Med. 1970 May 14;282(20):1128-38. doi: 10.1056/NEJM197005142822005.
9
Homeoviscous adaptation--a homeostatic process that regulates the viscosity of membrane lipids in Escherichia coli.同型粘性适应——一种调节大肠杆菌膜脂粘度的稳态过程。
Proc Natl Acad Sci U S A. 1974 Feb;71(2):522-5. doi: 10.1073/pnas.71.2.522.
10
A spin probe study of the influence of cholesterol on motion and orientation of phospholipids in oriented multibilayers and vesicles.
Chem Phys Lipids. 1973 Jan;10(1):11-27. doi: 10.1016/0009-3084(73)90037-6.
Zotero 插件