来自胆固醇生物合成调节缺陷的体细胞突变体的质膜磷脂组成的适应性改变。
Adaptive alteration in phospholipid composition of plasma membranes from a somatic cell mutant defective in the regulation of cholesterol biosynthesis.
作者信息
Sinensky M
出版信息
J Cell Biol. 1980 Apr;85(1):166-9. doi: 10.1083/jcb.85.1.166.
Abstract
A somatic cell mutant (CR1) of a Chinese hamster ovary cell (CHO-K1) which has previously been shown to be defective in the regulation of cholesterol biosynthesis accumulates more cholesterol than the parental cell line in plasma membranes. Although such an increase in membrane cholesterol should lead to an increase in the order parameter of these membranes, as measured with an electron spin resonance spin probe, the order parameters of mutant and wild-type plasma membranes are identical--apparently because of an adaptive alteration in membrane phospholipid composition. The phospholipid compositions of mutant and wild-type cell plasma membranes are compared and the mutant is shown to have a threefold higher level of oleic acid and a twofold lower level of phosphatidylethanolamine than the wild type. These results are consistent with model studies which show that these compositional changes lead to lower-order parameters for phospholipid dispersions.
摘要
中国仓鼠卵巢细胞（CHO-K1）的一个体细胞突变体（CR1），先前已证明其在胆固醇生物合成调节方面存在缺陷，该突变体在质膜中积累的胆固醇比亲本细胞系更多。尽管通过电子自旋共振自旋探针测量，膜胆固醇的这种增加应导致这些膜的序参数增加，但突变体质膜和野生型质膜的序参数是相同的——这显然是由于膜磷脂组成的适应性改变。比较了突变体和野生型细胞质膜的磷脂组成，结果表明，与野生型相比，突变体的油酸水平高三倍，磷脂酰乙醇胺水平低两倍。这些结果与模型研究一致，模型研究表明这些组成变化会导致磷脂分散体的序参数降低。
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