Rasheed Sultana, Bashir Khurram, Nakaminami Kentaro, Hanada Kousuke, Matsui Akihiro, Seki Motoaki
a Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource Sciences , Suehiro-cho, Tsurumi-ku, Yokohama , Kanagawa , Japan.
b Kihara Institute for Biological Research, Yokohama City University , Yokohama , Japan.
Plant Signal Behav. 2016 Aug 2;11(8):e1215792. doi: 10.1080/15592324.2016.1215792.
Characterizing the molecular mechanisms governing the response of plant roots and shoots to drought stress could aid the development of strategies aiming to ameliorate drought stress. Small open reading frames (sORFs), putatively encoding small peptides, may play a significant role in the response to different abiotic stresses. Microarray analyses revealed that after 5, 7 and 9 d of a drought treatment, 2, 77, and 104 sORFs were up-regulated in roots, respectively; while the number of upregulated sORFs in shoots was 12, 45, and 158, respectively. RT-qPCR analysis confirmed the up-regulated expression of ATRIKEN29196 and ATRIKEN32280 specifically in roots. The identified upregulated sORFs, particularly those in roots, may contribute to drought stress tolerance.
阐明植物根和地上部分对干旱胁迫响应的分子机制,有助于制定旨在缓解干旱胁迫的策略。推测编码小肽的小开放阅读框(sORF)可能在对不同非生物胁迫的响应中发挥重要作用。微阵列分析显示,干旱处理5、7和9天后,根中分别有2、77和104个sORF上调;而地上部分上调的sORF数量分别为12、45和158个。RT-qPCR分析证实了ATRIKEN29196和ATRIKEN32280仅在根中上调表达。鉴定出的上调sORF,尤其是根中的那些,可能有助于提高对干旱胁迫的耐受性。