College of Tobacco Science, Henan Agricultural University, 63 Nongye Road, Zhengzhou, Henan Province 450002, PR China.
College of Tobacco Science, Henan Agricultural University, 63 Nongye Road, Zhengzhou, Henan Province 450002, PR China.
J Photochem Photobiol B. 2016 Sep;162:544-549. doi: 10.1016/j.jphotobiol.2016.07.016. Epub 2016 Jul 20.
Flavonoids are important secondary metabolites in plants regulated by the environment. To analyze the effect of light quality on the accumulation of flavonoids, we performed a rapid analysis of flavonoids in extracts of tobacco leaves using UHPLC-QTOF. A total of 12 flavonoids were detected and identified in tobacco leaves, which were classified into flavonoid methyl derivatives and flavonoid glycoside derivatives according to the groups linked to the flavonoid core. Correlation analysis was further conducted to investigate the effect of different wavelengths of light on their accumulation. The content of flavonoid methyl derivatives was positively correlated with the proportions of far-red light (FR; 716-810nm) and near-infrared light (NIR; 810-2200nm) in the sunlight spectrum and negatively correlated with the proportion of ultraviolet (UV-A; 350-400nm) and the red/far-red ratio (R/FR). By contrast, the content of flavonoid glycoside derivatives was positively correlated with the proportion of UV-A and the R/FR, and negatively correlated with FR and NIR. The results indicated that light quality with higher proportions of FR and NIR increases the activity of flavonoid methyltransferases but suppresses the activity of flavonoid glycoside transferases. While a high proportion of UV-A and a high R/FR can increase flavonoid glycoside transferase activity but suppress flavonoid methyltransferase activity.
类黄酮是植物中受环境调控的重要次生代谢物。为了分析光质对类黄酮积累的影响，我们使用 UHPLC-QTOF 对烟草叶片提取物中的类黄酮进行了快速分析。共检测到 12 种类黄酮，并根据与类黄酮核心相连的基团将其分为类黄酮甲基衍生物和类黄酮糖苷衍生物。进一步进行了相关分析，以研究不同波长的光对其积累的影响。类黄酮甲基衍生物的含量与太阳光谱中远红（716-810nm）和近红外（810-2200nm）光的比例呈正相关，与紫外（UV-A；350-400nm）光的比例和红/远红比（R/FR）呈负相关。相比之下，类黄酮糖苷衍生物的含量与 UV-A 和 R/FR 的比例呈正相关，与 FR 和 NIR 呈负相关。结果表明，FR 和 NIR 比例较高的光质会增加类黄酮甲基转移酶的活性，但会抑制类黄酮糖苷转移酶的活性。而高比例的 UV-A 和高的 R/FR 可以增加类黄酮糖苷转移酶的活性，但抑制类黄酮甲基转移酶的活性。
