Institute of Biomedical Materials and Engineering, College of Materials Science and Engineering, Qingdao University, Qingdao 266071, China.
Institute of Biomedical Materials and Engineering, College of Materials Science and Engineering, Qingdao University, Qingdao 266071, China; Laboratory for New Fiber Materials and Modern Textile, Growing Base for State Key Laboratory, College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, China.
Talanta. 2018 Oct 1;188:493-498. doi: 10.1016/j.talanta.2018.06.027. Epub 2018 Jun 9.
Recently, the separation of proteins has received much attention, although many techniques require expensive instrumentation and trained analysts. In this work, a low-cost, effective, and environmental friendship capillary electrophoresis (CE) for proteins separation was first time introduced. The ZIF-8 with outstanding properties of large surface area, and accessible tunnels and cages were coated the inner surface of silica capillary as a separation media by electrostatic interaction. The fast baseline separation of Lys, CC, BSA and RNase A can be obtained within 10 min using the ZIF-8 nanocrystals coated capillary column under the optimum separation conditions. Meanwhile, this system showed good reproducibility and stability. Using L-glutamic acid as the selector ligand, the D- and L-phenylalanine were successfully separated by the ZIF-8 nanocrystals coated capillary column. Furthermore, the method was also applied to separate egg white proteins, and three main proteins were separated in a single run.
最近，蛋白质的分离受到了广泛关注，尽管许多技术都需要昂贵的仪器和训练有素的分析人员。在这项工作中，首次提出了一种低成本、有效且环保的用于蛋白质分离的毛细管电泳（CE）。ZIF-8 具有大的比表面积、可及的隧道和笼的优异性质，通过静电相互作用将其涂覆在硅胶毛细管的内表面作为分离介质。在最佳分离条件下，使用 ZIF-8 纳米晶体涂覆的毛细管柱可在 10 min 内快速基线分离赖氨酸、CC、BSA 和 RNase A。同时，该系统表现出良好的重现性和稳定性。使用 L-谷氨酸作为选择配体，通过 ZIF-8 纳米晶体涂覆的毛细管柱成功分离了 D-和 L-苯丙氨酸。此外，该方法还应用于分离蛋清蛋白，可在一次运行中分离三种主要蛋白质。
