Plumeré Nicolas, Nowaczyk Marc M
Ruhr-University Bochum, Bochum, Germany.
Adv Biochem Eng Biotechnol. 2016;158:111-136. doi: 10.1007/10_2016_7.
This chapter presents biophotoelectrochemical systems where one of nature's photosynthetic proteins, such as photosystem 1 (PS1), photosystem 2 (PS2), or bacterial reaction centers, are employed to create devices for technological applications. We use recent advances in biophotoelectrodes for energy conversion and sensing to illustrate the fundamental approaches in half-cell design and characterization. The aim is to guide electrochemists and photosynthetic researchers in the development of hybrid systems interfacing photosynthetic proteins with electrodes ranging from biosensors to biophotovoltaic cells. The first part gives an overview of the photosynthetic electron transfer chain with details on photosynthetic proteins and on the properties relevant for technological applications. The second part describes and critically discusses the main applications of biophotoelectrochemical cells based on photosynthetic proteins and exposes the respective requirement in electrode design. The following and final parts present the standard methodologies for the characterization of the biophotoelectrochemical half-cells with the main objectives of enhancing our mechanistic understanding of electron transfer, charge recombination, overpotential in photocurrent generation and protein degradation processes in devices, and thus open the perspectives for novel biophotoelectrochemical concepts and their rational optimization toward practical efficiencies.
本章介绍了生物光电化学系统，其中利用自然界的光合蛋白之一，如光系统1（PS1）、光系统2（PS2）或细菌反应中心，来制造用于技术应用的装置。我们利用生物光电极在能量转换和传感方面的最新进展，来说明半电池设计和表征的基本方法。目的是指导电化学家和光合研究人员开发将光合蛋白与电极连接起来的混合系统，这些系统涵盖从生物传感器到生物光伏电池等。第一部分概述了光合电子传递链，详细介绍了光合蛋白以及与技术应用相关的特性。第二部分描述并批判性地讨论了基于光合蛋白的生物光电化学电池的主要应用，并阐述了电极设计中的相应要求。接下来的最后几部分介绍了生物光电化学半电池表征的标准方法，主要目的是增强我们对电子转移、电荷复合、光电流产生中的过电位以及装置中蛋白质降解过程的机理理解，从而为新型生物光电化学概念及其朝着实际效率的合理优化开辟前景。
