一步基质应用法用于 MALDI 质谱成像细菌菌落生物膜。
A one-step matrix application method for MALDI mass spectrometry imaging of bacterial colony biofilms.
机构信息
Department of Chemistry and Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
Department of Chemical and Biomolecular Engineering and Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.
出版信息
J Mass Spectrom. 2016 Nov;51(11):1030-1035. doi: 10.1002/jms.3827.
Abstract
Matrix-assisted laser desorption/ionization imaging of biofilms cultured on agar plates is challenging because of problems related to matrix deposition onto agar. We describe a one-step, spray-based application of a 2,5-dihydroxybenzoic acid solution for direct matrix-assisted laser desorption/ionization imaging of hydrated Bacillus subtilis biofilms on agar. Using both an optimized airbrush and a home-built automatic sprayer, region-specific distributions of signaling metabolites and cannibalistic factors were visualized from B. subtilis cells cultivated on biofilm-promoting medium. The approach provides a homogeneous, relatively dry coating on hydrated samples, improving spot to spot signal repeatability compared with sieved matrix application, and is easily adapted for imaging a range of agar-based biofilms. Copyright © 2016 John Wiley & Sons, Ltd.
摘要
基质辅助激光解吸/电离成像技术在琼脂平板上培养的生物膜中应用具有挑战性，因为与基质沉积到琼脂上有关的问题。我们描述了一种一步法，即用喷雾器将 2,5-二羟基苯甲酸溶液直接喷洒到琼脂上，用于对水合枯草芽孢杆菌生物膜进行直接基质辅助激光解吸/电离成像。使用优化的喷枪和自制的自动喷雾器，从在促进生物膜形成的培养基中培养的枯草芽孢杆菌细胞中可视化了信号代谢物和自噬因子的区域特异性分布。该方法在水合样品上提供了均匀的、相对干燥的涂层，与筛基质应用相比，提高了斑点间信号重复性，并且易于适应各种基于琼脂的生物膜成像。版权所有 © 2016 年 John Wiley & Sons, Ltd.
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