Asano N, Morita T, Watanabe Y
Biochemical Research Laboratory, Nitto Denko Corporation, Osaka, Japan.
Mutat Res. 1989 Aug;223(4):391-4. doi: 10.1016/0165-1218(89)90093-1.
The effects of micronucleus induction in mouse bone marrow cells by intraperitoneal injection (i.p.) and oral administration (p.o.) were studied with the spindle poison colchicine in 2 strains of mice (MS/Ae and CD-1). The final micronucleus test was performed with a 24-h sampling time at doses of 0.25-2.0 mg/kg by the i.p. route and 2-16 mg/kg by the p.o. route based on a simple acute toxicity test and a pilot micronucleus experiment. Colchicine induced micronuclei in both strains and at all doses tested. Micronuclei were induced more effectively by the i.p. route.
采用纺锤体毒物秋水仙碱，研究了腹腔注射（i.p.）和口服（p.o.）两种给药方式对2个品系小鼠（MS/Ae和CD-1）骨髓细胞微核诱导的影响。基于简单急性毒性试验和预试验微核实验，最终微核试验采用24小时采样时间，腹腔注射途径剂量为0.25 - 2.0毫克/千克，口服途径剂量为2 - 16毫克/千克。秋水仙碱在所有测试剂量下均能在两个品系中诱导微核形成。腹腔注射途径诱导微核的效果更显著。
